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인간 지방유래 줄기세포 엑소좀: 분리, 특성분석 및 기능성 화장품 소재로의 응용

Title
인간 지방유래 줄기세포 엑소좀: 분리, 특성분석 및 기능성 화장품 소재로의 응용
Other Titles
Exosomes from human adipose-derived stem cells: Isolation, characterization and application to functional cosmetics
Author
조우리
Advisor(s)
조용우
Issue Date
2017-02
Publisher
한양대학교
Degree
Master
Abstract
Stem cell-derived exosomes contain bioactive substances such as proteins, messenger RNAs, and microRNAs and play important role in tissue regeneration via the transfer of specific bioactive substances. Herein, we hypothesized that exosomes secreted by adipose-derived stem cells (HASCs) have a positive role in promotion of collagen remodeling in UVB-induced skin damage. Exosomes (P-EXOs) were isolated from proliferating HASCs through multi-filtration system (MFS) based on tangential flow filtration (TFF) and characterized by different methodologies. The P-EXOs contained various microRNAs and proteins related to skin development and extracellular matrix (ECM) synthesis. We found that P-EXOs significantly promoted proliferation, migration and collagen synthesis of human fibroblasts, with increased gene expression of tissue inhibitor of megalloproteinase-1/-3 (TIMP-1/-3) and collagen type I alpha 1 (COL1A1). Our findings suggest that P-EXOs act as a biological cue stimulating dermal fibroblasts and provide a new approach for functional cosmetics in skin rejuvenation.
URI
http://dcollection.hanyang.ac.kr/jsp/common/DcLoOrgPer.jsp?sItemId=000000099743https://repository.hanyang.ac.kr/handle/20.500.11754/124565
Appears in Collections:
GRADUATE SCHOOL[S](대학원) > FUSION CHEMICAL ENGINEERING(융합화학공학과) > Theses (Master)
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