Feasibility study for combination of field-flow fractionation (FFF)-based separation of size-coded particle probes with amplified surface enhanced Raman scattering (SERS) tagging for simultaneous detection of multiple miRNAs
- Title
- Feasibility study for combination of field-flow fractionation (FFF)-based separation of size-coded particle probes with amplified surface enhanced Raman scattering (SERS) tagging for simultaneous detection of multiple miRNAs
- Author
- 정회일
- Keywords
- Gravitational field-flow fractionation (GrFFF); Surface-enhanced Raman scattering (SERS); MicroRNAs; Size-coded probes; Polyadenylation; Multiplexed detection
- Issue Date
- 2018-06
- Publisher
- ELSEVIER SCIENCE BV
- Citation
- JOURNAL OF CHROMATOGRAPHY A, v. 1556, page. 97-102
- Abstract
- We propose a new analytical scheme in which field-flow fractionation (FFF)-based separation of target-specific polystyrene (PS) particle probes of different sizes are incorporated with amplified surface enhanced Raman scattering (SERS) tagging for the simultaneous and sensitive detection of multiple microRNAs (miRNAs). For multiplexed detection, PS particles of three different diameters (15, 10, 5 mu m) were used for the size-coding, and a probe single stranded DNA (ss DNA) complementary to a target miRNA was conjugated on an intended PS particle. After binding of a target miRNA on PS probe, polyadenylation reaction was executed to generate a long tail composed of adenine (A) serving as a binding site to thymine (T) conjugated Au nanoparticles (T-AuNPs) to increase SERS intensity. The three size-coded PS probes bound with T-AuNPs were then separated in a FFF channel. With the observation of extinction based fractograms, separation of three size-coded PS probes was clearly confirmed, thereby enabling of measuring three miRNAs simultaneously. Raman intensities of FFF fractions collected at the peak maximum of 15, 10 and 5 mu m PS probes varied fairy quantitatively with the change of miRNA concentrations, and the reproducibility of measurement was acceptable. The proposed method is potentially useful for simultaneous detection of multiple miRNAs with high sensitivity.
- URI
- https://www.sciencedirect.com/science/article/pii/S0021967318305181?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/118958
- ISSN
- 0021-9673; 1873-3778
- DOI
- 10.1016/j.chroma.2018.04.057
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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