Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤문영 | - |
dc.date.accessioned | 2019-12-08T01:43:02Z | - |
dc.date.available | 2019-12-08T01:43:02Z | - |
dc.date.issued | 2018-05 | - |
dc.identifier.citation | ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, v. 646, page. 16-23 | en_US |
dc.identifier.issn | 0003-9861 | - |
dc.identifier.issn | 1096-0384 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/abs/pii/S0003986117306562?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/118632 | - |
dc.description.abstract | Anthrax is caused by Bacillus anthracis, a bacterium that is able to secrete the toxins protective antigen, edema factor and lethal factor. Due to the high level of secretion from the bacteria and its severe virulence, lethal factor (LF) has been sought as a biomarker for detecting bacterial infection and as an effective target to neutralize toxicity. In this study, we found three aptamers, and binding affinity was determined by fluorescently labeled aptamers. One of the aptamers exhibited high affinity, with a K-d value of 11.0 +/- 2.7 nM, along with low cross reactivity relative to bovine serum albumin and protective antigen. The therapeutic functionality of the aptamer was examined by assessing the inhibition of LF protease activity against a mitogen-activated protein kinase kinase. The aptamer appears to be an effective inhibitor of LF with an IC50 value of 15 +/- 1.5 mu M and approximately 85% cell viability, suggesting that this aptamer provides a potential clue for not only development of a sensitive diagnostic device of B. anthracis infection but also the design of novel inhibitors of LF. | en_US |
dc.description.sponsorship | This work was supported by the National Institute of General Medical Sciences of the National Institutes of Health under award number P20GM10344. This work was also in part supported by the Korean Science and Engineering Foundation and the Ministry of Education, Science and Technology through its National Technology Program (2009-0081812) and by the Cooperative Research Program grants for Agriculture Science & Technology Development (Project No. PJ907052) funded by the Rural Development Administration, Republic of Korea. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER SCIENCE INC | en_US |
dc.subject | IN-VITRO SELECTION | en_US |
dc.subject | PROTECTIVE ANTIGEN | en_US |
dc.subject | BACILLUS-ANTHRACIS | en_US |
dc.subject | DNA APTAMERS | en_US |
dc.subject | TOXIN | en_US |
dc.subject | QUADRUPLEXES | en_US |
dc.subject | BINDING | en_US |
dc.subject | RECEPTORS | en_US |
dc.subject | MOLECULES | en_US |
dc.subject | PATHWAYS | en_US |
dc.title | Inhibition of anthrax lethal factor by ssDNA aptamers | en_US |
dc.type | Article | en_US |
dc.relation.volume | 646 | - |
dc.identifier.doi | 10.1016/j.abb.2018.03.028 | - |
dc.relation.page | 16-23 | - |
dc.relation.journal | ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | - |
dc.contributor.googleauthor | Lahousse, Mieke | - |
dc.contributor.googleauthor | Park, Hae-Chul | - |
dc.contributor.googleauthor | Lee, Sang-Choon | - |
dc.contributor.googleauthor | Ha, Na-Reum | - |
dc.contributor.googleauthor | Jung, In-Pil | - |
dc.contributor.googleauthor | Schlesinger, Sara R. | - |
dc.contributor.googleauthor | Shackelford, Kaylin | - |
dc.contributor.googleauthor | Yoon, Moon-Young | - |
dc.contributor.googleauthor | Kim, Sung-Kun | - |
dc.relation.code | 2018002877 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF CHEMISTRY | - |
dc.identifier.pid | myyoon | - |
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