Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 배상수 | - |
dc.date.accessioned | 2019-12-06T06:11:21Z | - |
dc.date.available | 2019-12-06T06:11:21Z | - |
dc.date.issued | 2018-03 | - |
dc.identifier.citation | BIOINFORMATICS, v. 34, no. 6, page. 1077-1079 | en_US |
dc.identifier.issn | 1367-4803 | - |
dc.identifier.issn | 1460-2059 | - |
dc.identifier.uri | https://academic.oup.com/bioinformatics/article/34/6/1077/4665414 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/117917 | - |
dc.description.abstract | Following the type II CRISPR-Cas9 system, type V CRISPR-Cpf1 endonucleases have been found to be applicable for genome editing in various organisms in vivo. However, there are as yet no web-based tools capable of optimally selecting guide RNAs (gRNAs) among all possible genome-wide target sites. Here, we present Cpf1-Database, a genome-wide gRNA library design tool for LbCpf1 and AsCpf1, which have DNA recognition sequences of 5'-TTTN-3' at the 5' ends of target sites. Cpf1-Database provides a sophisticated but simple way to design gRNAs for AsCpf1 nucleases on the genome scale. One can easily access the data using a straightforward web interface, and using the powerful collections feature one can easily design gRNAs for thousands of genes in short time. | en_US |
dc.description.sponsorship | This study was supported by Next-Generation BioGreen 21 Program (PJ01119201) and by Korea Healthcare technology R&D Project (HI16C1012) and by Basic Science Research Program (NRF-2016R1D1A1B0 3934811) to S.B. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | OXFORD UNIV PRESS | en_US |
dc.subject | HUMAN-CELLS | en_US |
dc.subject | FUNCTIONAL GENOMICS | en_US |
dc.subject | TARGET SITES | en_US |
dc.subject | CPF1 | en_US |
dc.subject | SPECIFICITIES | en_US |
dc.subject | ENDONUCLEASES | en_US |
dc.subject | NUCLEASES | en_US |
dc.subject | SYSTEM | en_US |
dc.title | Cpf1-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cpf1 | en_US |
dc.type | Article | en_US |
dc.relation.no | 6 | - |
dc.relation.volume | 34 | - |
dc.identifier.doi | 10.1093/bioinformatics/btx695 | - |
dc.relation.page | 1077-1079 | - |
dc.relation.journal | BIOINFORMATICS | - |
dc.contributor.googleauthor | Park, Jeongbin | - |
dc.contributor.googleauthor | Bae, Sangsu | - |
dc.relation.code | 2018000980 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF CHEMISTRY | - |
dc.identifier.pid | sangsubae | - |
dc.identifier.researcherID | E-5324-2017 | - |
dc.identifier.orcid | http://orcid.org/0000-0003-3615-8566 | - |
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