Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Hyunsook Kim | - |
dc.date.accessioned | 2019-12-04T02:32:23Z | - |
dc.date.available | 2019-12-04T02:32:23Z | - |
dc.date.issued | 2018-01 | - |
dc.identifier.citation | POULTRY SCIENCE, v. 97, no. 1, page. 289-293 | en_US |
dc.identifier.issn | 0032-5791 | - |
dc.identifier.issn | 1525-3171 | - |
dc.identifier.uri | https://academic.oup.com/ps/article/97/1/289/4590019 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/117089 | - |
dc.description.abstract | Overgrowth of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) on Campylobacter media prevents the latter's selective isolation, thereby making the improvement of Campylobacter-selective media necessary. We evaluated tazobactam (an ESBL inhibitor) to supplement Bolton enrichment broth (Tz-Bolton broth) for the selective isolation of Campylobacter in chicken carcass rinses. First, using 20 strains of ESBL-producing E. coli and 13 Campylobacter strains, we found 4 mu g/mL of tazobactam to be optimal for inhibiting the ESBL-producing E. coli while allowing the growth of all tested Campylobacter strains. Next, 80 whole chicken carcasses were rinsed with buffered peptone water (BPW), and 25 mL of BPW rinse was mixed with 2 x blood-free Bolton broth (25 mL) with or without tazobactam followed by incubation at 42 degrees C for 48 h under microaerobic conditions. A loopful of the incubated broth was inoculated on modified charcoal-cefoperazone-deoxycholate agar (mCCDA) and microaerobically incubated at 42 degrees C for 48 h. The tazobactam supplemented Bolton broth showed a higher Campylobacter isolation rate (38.8%, p < 0.05) than normal Bolton broth (15%). Moreover, the number of mCCDA plates with non-Campylobacter was much lower (p < 0.05) after enrichment in Tz-Bolton broth (0%) than in the normal Bolton broth (80%), suggesting that selectivity of the modified broth was superior to normal Bolton broth. | en_US |
dc.description.sponsorship | We thank Drs. Carl Cerniglia, Fatemeh Rafii, and John Sutherland for critical review of the manuscript. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2015R1A2A2A05001288). The views expressed herein do not necessarily reflect those of the US Food and Drug Administration or the US Department of Health and Human Services. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | OXFORD UNIV PRESS | en_US |
dc.subject | Campylobacter | en_US |
dc.subject | Tazobactam | en_US |
dc.subject | Bolton broth | en_US |
dc.subject | Chicken | en_US |
dc.title | Improvement of Bolton broth by supplementation with tazobactam for the isolation of Campylobacter from chicken rinses | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 97 | - |
dc.identifier.doi | 10.3382/ps/pex169 | - |
dc.relation.page | 289-293 | - |
dc.relation.journal | POULTRY SCIENCE | - |
dc.contributor.googleauthor | Chon, Jung-Whan | - |
dc.contributor.googleauthor | Kim, Young-Ji | - |
dc.contributor.googleauthor | Rashid, Farzana | - |
dc.contributor.googleauthor | Sung, Kidon | - |
dc.contributor.googleauthor | Khan, Saeed | - |
dc.contributor.googleauthor | Kim, Hyunsook | - |
dc.contributor.googleauthor | Seo, Kun-Ho | - |
dc.relation.code | 2018001898 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF HUMAN ECOLOGY[S] | - |
dc.sector.department | DEPARTMENT OF FOOD & NUTRITION | - |
dc.identifier.pid | hyunsk15 | - |
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