Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 상병인 | - |
dc.date.accessioned | 2019-11-30T16:10:32Z | - |
dc.date.available | 2019-11-30T16:10:32Z | - |
dc.date.issued | 2017-09 | - |
dc.identifier.citation | BIOTECHNOLOGY FOR BIOFUELS, v. 10, Article no. 212 | en_US |
dc.identifier.issn | 1754-6834 | - |
dc.identifier.uri | https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-017-0900-3 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/115566 | - |
dc.description.abstract | Background: In the biorefinery utilizing lignocellulosic biomasses, lignin decomposition to value-added phenolic derivatives is a key issue, and recently biocatalytic delignification is emerging owing to its superior selectivity, low energy consumption, and unparalleled sustainability. However, besides heme-containing peroxidases and laccases, information about lignolytic biocatalysts is still limited till date. Results: Herein, we report a promiscuous activity of tyrosinase which is closely associated with delignification requiring high redox potentials (>1.4 V vs. normal hydrogen electrode [NHE]). The promiscuous activity of tyrosinase not only oxidizes veratryl alcohol, a commonly used nonphenolic substrate for assaying ligninolytic activity, to veratraldehyde but also cleaves the 4-O-5 and C-alpha-C-beta bonds in 4-phenoxyphenol and guaiacyl glycerol-beta-guaiacyl ether (GGE) that are dimeric lignin model compounds. Cyclic voltammograms additionally verified that the promiscuous activity oxidizes lignin-related high redox potential substrates. Conclusion: These results might be applicable for extending the versatility of tyrosinase toward biocatalytic delignification as well as suggesting a new perspective for sustainable lignin utilization. Furthermore, the results provide insight for exploring the previously unknown promiscuous activities of biocatalysts much more diverse than ever thought before, thereby innovatively expanding the applicable area of biocatalysis. | en_US |
dc.description.sponsorship | This work was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science, and Technology (NRF-2009-C1AAA001-0093286), and Korea Evaluation Institution of Industrial Technology funded by Ministry of Trade, Industry, and Energy (10049675). This work was also supported by the National Research Council of Science and Technology (NST) granted by the Korean government (MSIP) (No. CAP-11-04-KIST). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | BIOMED CENTRAL LTD | en_US |
dc.subject | Sustainable lignin utilization | en_US |
dc.subject | Tyrosinase | en_US |
dc.subject | Promiscuous activity | en_US |
dc.subject | 4-Phenoxyphenol | en_US |
dc.subject | Guaiacyl glycerol-beta-guaiacyl ether (GGE) | en_US |
dc.title | Perspectives for biocatalytic lignin utilization: cleaving 4-O-5 and C-alpha-C-beta bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinase | en_US |
dc.type | Article | en_US |
dc.relation.volume | 10 | - |
dc.identifier.doi | 10.1186/s13068-017-0900-3 | - |
dc.relation.page | 212-212 | - |
dc.relation.journal | BIOTECHNOLOGY FOR BIOFUELS | - |
dc.contributor.googleauthor | Min, Kyoungseon | - |
dc.contributor.googleauthor | Yum, Taewoo | - |
dc.contributor.googleauthor | Kim, Jiye | - |
dc.contributor.googleauthor | Woo, Han Min | - |
dc.contributor.googleauthor | Kim, Yunje | - |
dc.contributor.googleauthor | Sang, Byoung-In | - |
dc.contributor.googleauthor | Yoo, Young Je | - |
dc.contributor.googleauthor | Kim, Yong Hwan | - |
dc.contributor.googleauthor | Um, Youngsoon | - |
dc.relation.code | 2017008439 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF CHEMICAL ENGINEERING | - |
dc.identifier.pid | biosang | - |
dc.identifier.researcherID | T-2817-2017 | - |
dc.identifier.orcid | http://orcid.org/0000-0001-7972-6709 | - |
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