Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김동립 | - |
dc.date.accessioned | 2019-11-04T05:35:54Z | - |
dc.date.available | 2019-11-04T05:35:54Z | - |
dc.date.issued | 2019-05 | - |
dc.identifier.citation | ACS APPLIED MATERIALS & INTERFACES, v. 11, Page. 18968-18977 | en_US |
dc.identifier.issn | 1944-8244 | - |
dc.identifier.issn | 1944-8252 | - |
dc.identifier.uri | https://pubs.acs.org/doi/10.1021/acsami.9b03420 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/111795 | - |
dc.description.abstract | A viable approach for methanol production under ambient physiological conditions is to use greenhouse gases, methane (CH4) and carbon dioxide (CO2), as feed for immobilized methanotrophs. In the present study, unique macroporous carbon particles with pore sizes in the range of similar to 1-6 mu m were synthesized and used as support for the immobilization of Methylocella tundrae. Immobilization was accomplished covalently on hierarchical macro porous carbon particles. Maximal cell loading of covalently immobilized M. tundrae was 205 mg(DCM) g(-1) of particles. Among these particles, the cells immobilized on 3.6 mu m pore size particles showed the highest reusability with the least leaching and were chosen for further study. After immobilization, M. tundrae showed up to 2.4 -fold higher methanol production stability at various pH and temperature values because of higher stability and metabolic activity than free cells. After eight cycles of reuse, the immobilized cells retained 18.1 -fold higher relative production stability compared to free cells. Free and immobilized cells exhibited cumulative methanol production of 5.2 and 9.5 mu mol mg(DCM)(-1) under repeated batch conditions using simulated biogas [CH4 and CO2, 4:1 (v/v)] as feed, respectively. The appropriate pore size of macroporous particles favors the efficient M. tundrae immobilization to retain better biocatalytic properties. This is the first report concerning the covalent immobilization of methanotrophs on the newly synthesized macroporous carbon particles and its subsequent application in repeated methanol production using simulated biogas as a feed. | en_US |
dc.description.sponsorship | This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2017R1A2B3011676, 2017R1A4A1014806, 2013M3A6A8073184). This work was supported by WTU joint research grants and KU Research Professor program of Konkuk University. This study was also supported by the Intelligent Synthetic Biology Center of Global Frontier Project funded by the Ministry of Science and ICT of Korea (NRF-2012M3A6A8054889). | en_US |
dc.language.iso | en | en_US |
dc.publisher | AMER CHEMICAL SOC | en_US |
dc.subject | greenhouse gas | en_US |
dc.subject | immobilization | en_US |
dc.subject | macroporous particles | en_US |
dc.subject | methanol | en_US |
dc.subject | Methylocella tundrae | en_US |
dc.subject | simulated biogas | en_US |
dc.title | Hierarchical Macroporous Particles for Efficient Whole-Cell Immobilization: Application in Bioconversion of Greenhouse Gases to Methanol | en_US |
dc.type | Article | en_US |
dc.relation.volume | 11 | - |
dc.identifier.doi | 10.1021/acsami.9b03420 | - |
dc.relation.page | 18968-18977 | - |
dc.relation.journal | ACS APPLIED MATERIALS & INTERFACES | - |
dc.contributor.googleauthor | Patel, Sanjay K. S. | - |
dc.contributor.googleauthor | Jeon, Min Soo | - |
dc.contributor.googleauthor | Gupta, Rahul K. | - |
dc.contributor.googleauthor | Jeon, Yale | - |
dc.contributor.googleauthor | Kalia, Vipin Chandra | - |
dc.contributor.googleauthor | Kim, Sun Chang | - |
dc.contributor.googleauthor | Cho, Byung Kwan | - |
dc.contributor.googleauthor | Kim, Dong Rip | - |
dc.contributor.googleauthor | Lee, Jung-Kul | - |
dc.relation.code | 2019002549 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DIVISION OF MECHANICAL ENGINEERING | - |
dc.identifier.pid | dongrip | - |
dc.identifier.orcid | http://orcid.org/0000-0001-6398-9483 | - |
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