Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 전병훈 | - |
dc.date.accessioned | 2019-09-17T00:21:52Z | - |
dc.date.available | 2019-09-17T00:21:52Z | - |
dc.date.issued | 2019-03 | - |
dc.identifier.citation | JOURNAL OF BIOSCIENCE AND BIOENGINEERING, v. 127, NO 3, Page. 388-393 | en_US |
dc.identifier.issn | 1389-1723 | - |
dc.identifier.issn | 1347-4421 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S1389172318305164?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/110443 | - |
dc.description.abstract | Densitometric high performance thin layer chromatography (HPTLC) quantification method was developed to validate the decolorization/biotransformation of Disperse Orange ERL and dye mixture by lichen Parmelia sp. which release several colored compounds during decolorization process, hence unable to use colorimetric estimation. Percent decolorization of Disperse Orange ERL and dye mixture by lichen Parmelia sp. was observed when estimated using developed HPTLC method. Limit of detection and limit of quantification for both dyes in mixture were obtained as 03 and 1 mu g/mu l, respectively. Area of peak of control Disperse Orange ERL was reduced by 43% after 12 h, 71% after 48 h and upto 82% after 72 h of incubation. Precision and repeatability of data elucidated the % relative standard deviation less than 3 for all the values thus indicating statistically acceptable. Biodegradation of dye and mixture was confirmed with Fourier transform infrared spectroscopy analysis, i.e., altered fingerprinting spectral pattern. (C) 2018, The Society for Biotechnology, Japan. All rights reserved. | en_US |
dc.description.sponsorship | Ashwini N. Kulkarni is grateful to University Grants Commission for providing financial support in the form of NET-JRF, University Grants Commission, New Delhi. Bhumika N. Bhalkar is thankful to Council of Scientific and Industrial Research for providing CSIR-NET-SRF. Ashwini Kulkarni is grateful to Prof. D. K. Upreti, Lichenologist of National Botanical Research Institute, Lucknow, India for technical support. Authors are also thankful to IIT-SAIF for technical help. | en_US |
dc.language.iso | en | en_US |
dc.publisher | SOC BIOSCIENCE BIOENGINEERING JAPAN | en_US |
dc.subject | Quantification | en_US |
dc.subject | Densitometry | en_US |
dc.subject | High performance thin layer chromatography | en_US |
dc.subject | Silica gel | en_US |
dc.subject | Biodegradation | en_US |
dc.title | Densitometric quantification for the validation of decolorization of Disperse Orange ERL by lichen Parmelia sp | en_US |
dc.type | Article | en_US |
dc.relation.volume | 127 | - |
dc.identifier.doi | 10.1016/j.jbiosc.2018.08.001 | - |
dc.relation.page | 388-393 | - |
dc.relation.journal | JOURNAL OF BIOSCIENCE AND BIOENGINEERING | - |
dc.contributor.googleauthor | Kulkarni, Ashwini N. | - |
dc.contributor.googleauthor | Bhalkar, Bhumika N. | - |
dc.contributor.googleauthor | Khandare, Rahul V. | - |
dc.contributor.googleauthor | Kurade, Mayur B. | - |
dc.contributor.googleauthor | Jeon, Byong-Hun | - |
dc.contributor.googleauthor | Govindwar, Sanjay P. | - |
dc.relation.code | 2019000019 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF EARTH RESOURCES AND ENVIRONMENTAL ENGINEERING | - |
dc.identifier.pid | bhjeon | - |
dc.identifier.orcid | https://orcid.org/0000-0002-5478-765X | - |
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