시료주입시 기포발생이 억제된 반응조 형태의 중합효소연쇄반응용 PDMS/유리 바이오칩

Abstract

This paper reports low-cost microreactor (10 ㎕) biochip for the DNA PCR (polymerase chain reaction). The microbiochip (20㎜ × 28㎜) is a hybrid type which is composed of PDMS (polydimethylsiloxane) layer with serpentine micochannel (360 ㎛ × 100 ㎛) chamber and glass substrate integrated with microheater and thermal microsensor. Undesirable bubble is usually created during sample loading to PMDS-based microchip because of hydrophobic chip surface. Created bubbles interrupt stable biochemical reaction. We designed improved microreactor chamber using microfluidic simulation. The designed reactor has a cornerrounded serpentine channel architecture, which enables stable injection into hydrophobic surface using micropipette only. Reactor temperature needed to PCR reaction is controlled within ±0.5℃ by PID controller of LabVIEW software. It is experimentally confirmed that SRY gene PCR by the fabricated microreactor chip is performed for less than 54 min.