Simultaneous production of methane and acetate by thermophilic mixed culture from carbon dioxide in bioelectrochemical system

Abstract

A thermophilic bioelectrochemical system was operated with mixed culture at 60 °C, while introducing only carbon dioxide. Methane production was initially observed in a membrane-less single chamber without a mediator, but eventually acetate was also found as 10.5 g/L after 137 days of operation. Comparing the microbial communities before and after the electricity supply using next-generation sequencing technology, acetoclastic methanogens such as Methanosaeta concilii were increased, and this result also indicates the production of acetate in bioelectrochemical CO2 conversion system. With the advent of sulfate-reducing bacteria, Desulfotomaculum peckii was considered to be an acetate production promoter. These high production results for both methane and acetate can be applied to CO2 storage using excess electricity for value-added chemicals.