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Protective effect of MOTILIPERM in varicocele-induced oxidative injury in rat testis by activating phosphorylated inositol requiring kinase 1 alpha (p-IRE1 alpha) and phosphorylated c-Jun N-terminal kinase (p-JNK) pathways

Title
Protective effect of MOTILIPERM in varicocele-induced oxidative injury in rat testis by activating phosphorylated inositol requiring kinase 1 alpha (p-IRE1 alpha) and phosphorylated c-Jun N-terminal kinase (p-JNK) pathways
Author
김철영
Keywords
Sperm; oxidative stress; ER stress; testosterone
Issue Date
2018-01
Publisher
TAYLOR & FRANCIS LTD
Citation
PHARMACEUTICAL BIOLOGY, v. 56, No. 1, Page. 94-103
Abstract
Context: MOTILIPERM was prepared as a mixture of extracts of three medicinal herbs [roots of Morinda officinalis How (Rubiaceae), outer scales of Allium cepa L. (Liliaceae) and seeds of Cuscuta chinensis Lamark (Convolvulaceae)]. Objective: To investigate the role of reactive oxygen species (ROS)-based endoplasmic reticulum (ER) stress in a rat model of varicocele and the therapeutic efficacy of MOTILIPERM in this model. Materials and methods: Sixty male rats were divided into five experimental groups: a normal control group (CTR+vehicle), a control group administered MOTILIPERM 200 mg/kg (CTR+M 200), a varicocele-induced control group (VC+ vehicle) and two varicocele-induced groups administered MOTILIPERM 100 (VC+M 100) or 200 (VC+M 200) mg/kg for 4 weeks. Testis weights were recorded and serums were assayed for hormone concentrations. Tissues were subjected to semen analysis, histopathology, analyses of ER response protein expression levels and oxidative stress were assessed by measuring ROS, reactive nitrogen species (RNS), malondialdehyde (MDA) level and ratios of total glutathione (GSH)/oxidized GSH (GSSG). Results: MOTILIPERM treatment of varicocele-induced groups significantly increased left testis weight, testosterone level, sperm motility, count and spermatogenic cell density. ER-response protein expression levels were dose-dependently decreased in VC+M 200 group compared with VC+vehicle group. MOTILIPERM treatment also decreased MDA and ROS/RNS level but increased GSH/GSSG ratio. Discussion and conclusions: This study suggests that ROS-related ER stress may play a major role in varicocele-induced infertility and MOTILIPERM, a novel compound targeting ROS-based ER stress, may be therapeutically useful in treatment of varicocele, or as a supplement for the treatment of infertility.
URI
https://www.tandfonline.com/doi/abs/10.1080/13880209.2017.1421672https://repository.hanyang.ac.kr/handle/20.500.11754/80903
ISSN
1388-0209
DOI
10.1080/13880209.2017.1421672
Appears in Collections:
COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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