Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정승준 | - |
dc.date.accessioned | 2018-11-21T02:31:32Z | - |
dc.date.available | 2018-11-21T02:31:32Z | - |
dc.date.issued | 2016-09 | - |
dc.identifier.citation | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY, v. 20, NO. 5, Page. 525-531 | en_US |
dc.identifier.issn | 1226-4512 | - |
dc.identifier.issn | 2093-3827 | - |
dc.identifier.uri | https://synapse.koreamed.org/DOIx.php?id=10.4196/kjpp.2016.20.5.525 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/80557 | - |
dc.description.abstract | The analgesic mechanism of opioids is known to decrease the excitability of substantia gelatinosa (SG) neurons receiving the synaptic inputs from primary nociceptive afferent fiber by increasing inwardly rectifying K+ current. In this study, we examined whether a p-opioid agonist, [D-Ala2,N-Me-Phe4, Gly5-ol]-enkephalin (DAMGO), affects the two-pore domain K+ channel (K2P) current in rat SG neurons using a slice whole-cell patch clamp technique. Also we confirmed which subtypes of K2P channels were associated with DAMGO-induced currents, measuring the expression of K2P channel in whole spinal cord and SG region. DAMGO caused a robust hyperpolarization and outward current in the SG neurons, which developed almost instantaneously and did not show any time-dependent inactivation. Half of the SG neurons exhibited a linear I similar to V relationship of the DAMGO-induced current, whereas rest of the neurons displayed inward rectification. In SG neurons with a linear I similar to V relationship of DAMGO-induced current, the reversal potential was close to the K+ equilibrium potentials. The mRNA expression of TWIK (tandem of pore domains in a weak inwardly rectifying K+ channel) related acid-sensitive K+ channel (TASK) 1 and 3 was found in the SG region and a low pH (6.4) significantly blocked the DAMGO-induced K+ current. Taken together, the DAMGO-induced hyperpolarization at resting membrane potential and subsequent decrease in excitability of SG neurons can be carried by the two-pore domain K+ channel (TASK1 and 3) in addition to inwardly rectifying K+ channel. | en_US |
dc.description.sponsorship | This work was supported by the research fund of Hanyang University (HY-2012-N). | en_US |
dc.language.iso | en | en_US |
dc.publisher | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY | en_US |
dc.subject | DAMGO | en_US |
dc.subject | K+ current | en_US |
dc.subject | Opioid | en_US |
dc.subject | SG neuron | en_US |
dc.subject | TASK | en_US |
dc.title | DAMGO Modulates two-pore domain K+ channels in the substantia gelatinosa neurons of rat spinal cord | en_US |
dc.type | Article | en_US |
dc.relation.no | 5 | - |
dc.relation.volume | 20 | - |
dc.identifier.doi | 10.4196/kjpp.2016.20.5.525 | - |
dc.relation.page | 525-531 | - |
dc.relation.journal | KOREAN JOURNAL OF PHYSIOLOGY & PHARMACOLOGY | - |
dc.contributor.googleauthor | Cho, Pyung Sun | - |
dc.contributor.googleauthor | Lee, Han Kyu | - |
dc.contributor.googleauthor | Lee, Sang Hoon | - |
dc.contributor.googleauthor | Im, Jay Zoon | - |
dc.contributor.googleauthor | Jung, Sung Jun | - |
dc.relation.code | 2016004104 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | eurijj | - |
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