Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이근용 | - |
dc.date.accessioned | 2018-11-13T02:33:01Z | - |
dc.date.available | 2018-11-13T02:33:01Z | - |
dc.date.issued | 2016-09 | - |
dc.identifier.citation | NPG ASIA MATERIALS, v. 8, Page. 1-10 | en_US |
dc.identifier.issn | 1884-4049 | - |
dc.identifier.issn | 1884-4057 | - |
dc.identifier.uri | https://www.nature.com/articles/am2016130 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/80352 | - |
dc.description.abstract | Three-dimensional (3D) cell culture systems have promising applications compared with conventional two-dimensional cell culture systems. Herein, we report a facile method for the formation of 3D spheroids using novel thermo-reversible polysaccharide-based hydrogels. A series of thermo-reversible hydrogels consisting of N-acyl glycol chitosans (NAGCs) are synthesized through a simple N-acylation reaction, and the degree of acylation is finely tuned to obtain adequate thermo-reversible properties and gel stability. Among the NAGCs, N-hexanoyl glycol chitosan is the most thermo-sensitive and is highly effective for forming multi-cellular spheroids when used to coat the surfaces of cell culture dishes. Cell spheroids are effectively formed at various cell concentrations, and their spheroid shape and cellular functions are well maintained for longer times. The hydrogel culture system is also useful for co-cultures that mimic a biological microenvironment. Our thermo-reversible hydrogels may offer a convenient method for the development of in vitro 3D cell culture systems to provide enhanced performance in tissue regeneration, organ-on-chips, drug screening research and other biomedical applications. | en_US |
dc.description.sponsorship | This study was supported by an INNOPOLIS Foundation grant funded by the Korean government (Ministry of Science, ICT & Future Planning) through WINOVA (grant number 2014DD023), the Development of High Medical Technology Project (HI14C2755) of KHIDI, Korea, the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ009956), Rural Development Administration, Republic of Korea and National Research Foundation of korea grant funded by Korean Government (NRF-2016M3A9B4919616). This study was also partially supported by research funds awarded by Chungnam National University in 2016. We thank Eun Hee Han of the Korea Basic Science Institute (Daejeon, Republic of Korea) for her technical support in the confocal microscopy analysis. | en_US |
dc.language.iso | en | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.subject | HAIR FOLLICLE REGENERATION | en_US |
dc.subject | SPHEROIDS | en_US |
dc.subject | MEMBRANES | en_US |
dc.subject | COCULTURE | en_US |
dc.subject | MATRICES | en_US |
dc.subject | SYSTEMS | en_US |
dc.title | Bioinspired tuning of glycol chitosan for 3D cell culture | en_US |
dc.type | Article | en_US |
dc.relation.volume | 8 | - |
dc.identifier.doi | 10.1038/am.2016.130 | - |
dc.relation.page | 1-10 | - |
dc.relation.journal | NPG ASIA MATERIALS | - |
dc.contributor.googleauthor | Cho, Myeong Ok | - |
dc.contributor.googleauthor | Li, Zhengzheng | - |
dc.contributor.googleauthor | Shim, Hye-Eun | - |
dc.contributor.googleauthor | Cho, Ik-Sung | - |
dc.contributor.googleauthor | Nurunnabi, Md | - |
dc.contributor.googleauthor | Park, Honghyun | - |
dc.contributor.googleauthor | Lee, Kuen Yong | - |
dc.contributor.googleauthor | Moon, Sung-Hwan | - |
dc.contributor.googleauthor | Kim, Ki-Suk | - |
dc.contributor.googleauthor | Kang, Sun-Woong | - |
dc.contributor.googleauthor | Huh, Kang Moo | - |
dc.relation.code | 2016009160 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | leeky | - |
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