A Validated LC Method for Simultaneous Determination of Phenolic, Coumarin and Phthalide Compounds in the Ethanolic Extract of Angelica tenuissima
- Title
- A Validated LC Method for Simultaneous Determination of Phenolic, Coumarin and Phthalide Compounds in the Ethanolic Extract of Angelica tenuissima
- Author
- 유혜현
- Keywords
- Column liquid chromatography; Phenolic acids; Coumarins and phthalides; Angelica tenuissima
- Issue Date
- 2009-10
- Publisher
- VIEWEG
- Citation
- CHROMATOGRAPHIA, v. 70, No. 7-8, Page. 1079-1085
- Abstract
- In this study, an LC method for the simultaneous determination of six bioactive compounds from Angelica tenuissima, namely chlorogenic acid, ferulic acid, Z-ligustilide, nodakenin, decursin and decursinol angelate was developed and validated. Chromatographic analysis was carried out on a C(18) column with a mobile phase consisting of 0.1% formic acid, methanol and acetonitrile at a flow rate of 0.8 mL min(-1) and the effluent from the column was monitored by UV detector at 325 nm. The excellent linear behavior was observed over the investigated concentration range for reported compounds. The intra- and inter-day precision over the concentration range of compounds were lower than 1.7% (as relative standard deviation), and accuracy was between 97.2 and 106.0%. These results showed that the developed method is accurate, reproducible, and consequently applicable for the quantitation of bioactive components from the ethanolic extract of Angelica tenuissima Nakai.
- URI
- https://link.springer.com/article/10.1365%2Fs10337-009-1263-0https://repository.hanyang.ac.kr/handle/20.500.11754/76443
- ISSN
- 0009-5893
- DOI
- 10.1365/s10337-009-1263-0
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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