Changes in Bacillus anthracis CodY regulation under host-specific environmental factor deprived conditions
- Title
- Changes in Bacillus anthracis CodY regulation under host-specific environmental factor deprived conditions
- Author
- 채영규
- Keywords
- Bacillus anthracis; CodY; Environmental signals; Starvation; RNA-sequencing; Chromatin immunoprecipitation
- Issue Date
- 2016-08
- Publisher
- BIOMED CENTRAL LTD
- Citation
- BMC GENOMICS, v. 17, Page. 1-15
- Abstract
- Background: Host-specific environmental factors induce changes in Bacillus anthracis gene transcription during infection. A global transcription regulator, CodY, plays a pivotal role in regulating central metabolism, biosynthesis, and virulence in B. anthracis. In this study, we utilized RNA-sequencing to assess changes in the transcriptional patterns of CodY-regulated B. anthracis genes in response to three conditions of environmental starvation: iron, CO2, or glucose deprivation. In addition, we performed chromatin immunoprecipitation on newly identified CodY-mediated genes.
Results: Environmental deprivation induced transcriptional changes in CodY-regulated genes in both wild-type and codY strains, and both CodY-specific and environment-specific patterns were observed. In the iron-depleted condition, overexpression of iron homeostasis genes was observed independent of codY deletion; however, transcription of siderophore and amino acid biosynthesis genes was CodY dependent. Although CodY has a significant regulatory role in central metabolism and the carbon overflow pathway, metabolism-associated genes exhibited CodY-independent expression patterns under glucose starvation. Genes that were differentially expressed in response to CO2 availability showed CodY-dependent regulation, though their maximal expression did require a supply of CO2/bicarbonate.
Conclusions: We speculate that CodY regulates the expression of environmental-responsive genes in a hierarchical manner and is likely associated with other transcription regulators that are specific for a particular environmental change.
- URI
- https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-016-3004-8https://repository.hanyang.ac.kr/handle/20.500.11754/76402
- ISSN
- 1471-2164
- DOI
- 10.1186/s12864-016-3004-8
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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