Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황정욱 | - |
dc.date.accessioned | 2018-09-11T06:05:29Z | - |
dc.date.available | 2018-09-11T06:05:29Z | - |
dc.date.issued | 2016-08 | - |
dc.identifier.citation | SCIENTIFIC REPORTS, v. 6, Page. 1-13 | en_US |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://www.nature.com/articles/srep32252 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/75101 | - |
dc.description.abstract | The biological impact of alternative splicing is poorly understood in fungi, although recent studies have shown that these microorganisms are usually intron-rich. In this study, we re-annotated the genome of C. neoformans var. neoformans using RNA-Seq data. Comparison with C. neoformans var. grubii revealed that more than 99% of ORF-introns are in the same exact position in the two varieties whereas UTR-introns are much less evolutionary conserved. We also confirmed that alternative splicing is very common in C. neoformans, affecting nearly all expressed genes. We also observed specific regulation of alternative splicing by environmental cues in this yeast. However, alternative splicing does not appear to be an efficient method to diversify the C. neoformans proteome. Instead, our data suggest the existence of an intron retention-dependent mechanism of gene expression regulation that is not dependent on NMD. This regulatory process represents an additional layer of gene expression regulation in fungi and provides a mechanism to tune gene expression levels in response to any environmental modification. | en_US |
dc.description.sponsorship | This work was supported by National Research Foundation of Korea grants (2015R1A2A1A15055687) from MEST, the Strategic Initiative for Microbiomes in Agriculture and Food funded by Ministry of Agriculture, Food and Rural Affairs (916006-2) (to Y.S.B). This work was supported by a grant from ANR (2010-BLAN-1620-01 program YeastIntrons) to GJ. We thank Tae-Yup Kim and Anna Floyd for their technical assistance. We thank Cecelia Shertz Wall for editing the manuscript. | en_US |
dc.language.iso | en | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.subject | NONSENSE-MEDIATED DECAY | en_US |
dc.subject | MESSENGER-RNA DECAY | en_US |
dc.subject | DIFFERENTIAL EXPRESSION ANALYSIS | en_US |
dc.subject | NUCLEAR RETENTION | en_US |
dc.subject | YEAST | en_US |
dc.subject | GENOME | en_US |
dc.subject | SURVEILLANCE | en_US |
dc.subject | ARABIDOPSIS | en_US |
dc.subject | TRANSCRIPTOME | en_US |
dc.subject | VIRULENCE | en_US |
dc.title | Intron retention-dependent gene regulation in Cryptococcus neoformans | en_US |
dc.type | Article | en_US |
dc.relation.volume | 6 | - |
dc.identifier.doi | 10.1038/srep32252 | - |
dc.relation.page | 1-13 | - |
dc.relation.journal | SCIENTIFIC REPORTS | - |
dc.contributor.googleauthor | Gonzalez-Hilarion, Sara | - |
dc.contributor.googleauthor | Paulet, Damien | - |
dc.contributor.googleauthor | Lee, Kyung-Tae | - |
dc.contributor.googleauthor | Hon, Chung-Chau | - |
dc.contributor.googleauthor | Lechat, Pierre | - |
dc.contributor.googleauthor | Mogensen, Estelle | - |
dc.contributor.googleauthor | Moyrand, Frédérique | - |
dc.contributor.googleauthor | Proux, Caroline | - |
dc.contributor.googleauthor | Barboux, Rony | - |
dc.contributor.googleauthor | Hwang, Jungwook | - |
dc.relation.code | 2016012537 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jwhwang | - |
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