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Therapeutic effects of granulocyte-colony stimulating factor on non-alcoholic hepatic steatosis in the rat

Title
Therapeutic effects of granulocyte-colony stimulating factor on non-alcoholic hepatic steatosis in the rat
Author
송이선
Keywords
Sterol regulatory element-binding protein-1c; Otsuka Long Evans Tokushima Fatty; Non-alcoholic fatty liver disease; FATTY LIVER-DISEASE; G-CSF; MYOCARDIAL-INFARCTION; METABOLIC SYNDROME; OBESE MICE; OLETF RATS; EXERCISE; EXPRESSION; STEATOHEPATITIS; FIBROSIS
Issue Date
2013-01
Publisher
MEXICAN ASSOC HEPATOLOGY, PUNTE DE PIEDRA 150, COLONIA TORIELLO GUERRA, MEXICO, DF CP 14040, MEXICO
Citation
Annals of Hepatology, January 2013, 12(1), p.115-122
Abstract
Background and rationale. Non-alcoholic hepatic steatosis refers to the accumulation of triglycerides in the liver in the absence of alcohol consumption. Granulocyte colony-stimulating factor (G-CSF) has been reported to be an effective treatment for a variety of liver diseases. We examined the possible therapeutic effects of G-CSF on non-alcoholic hepatic steatosis in rats. Material and methods. Thirty-week-old Otsuka Long Evans Tokushima Fatty (OLETF) rats received water containing 30% sucrose for 8 weeks to promote the development of non-alcoholic hepatic steatosis. After development of the model, the rats were injected with G-CSF (100 mu g/kg/day) or saline for 5 days. Four weeks after this treatment, serum levels of glucose, total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and free fatty acids (FFA) were measured. Histology was examined by hematoxylin and eosin (H-E) and periodic acid Schiff (PAS) staining, and levels of expression of hepatic lipogenic enzymes were determined by RT-PCR. Results. The G-CSF-treated rats displayed significantly fewer lipid droplets than the saline-treated rats (P < 0.01), and their levels of sterol regulatory element-binding protein (SREBP)-1c, fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC) mRNAs were also lower (P < 0.01), as were their liver weight and serum levels of TG and FFA (P < 0.05). Conclusion. Our results indicate that G-CSF ameliorated non-alcoholic hepatic steatosis in the OLETF rat, and this therapeutic effect involved a reduction of SREBP-1c expression. Therefore, G-CSF deserves further study as a potential treatment for non-alcoholic hepatic steatosis.
URI
http://www.medigraphic.com/cgi-bin/new/resumen.cgi?IDARTICULO=59402https://repository.hanyang.ac.kr/handle/20.500.11754/74781
ISSN
1665-2681
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RESEARCH INSTITUTE[S](부설연구소) > ETC
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