Cas-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cas9
- Title
- Cas-Database: web-based genome-wide guide RNA library design for gene knockout screens using CRISPR-Cas9
- Author
- 배상수
- Keywords
- OFF-TARGET SITES; HUMAN-CELLS; FUNCTIONAL GENOMICS; TOOL; SYSTEM; CRISPR/CAS9; NUCLEASES
- Issue Date
- 2016-07
- Publisher
- OXFORD UNIV PRESS
- Citation
- BIOINFORMATICS (2016), v. 32, NO. 13, Page. 2017-2023
- Abstract
- Motivation: CRISPR-derived RNA guided endonucleases (RGENs) have been widely used for both gene knockout and knock-in at the level of single or multiple genes. RGENs are now available for forward genetic screens at genome scale, but single guide RNA (sgRNA) selection at this scale is difficult.
Results: We develop an online tool, Cas-Database, a genome-wide gRNA library design tool for Cas9 nucleases from Streptococcus pyogenes (SpCas9). With an easy-to-use web interface, Cas-Database allows users to select optimal target sequences simply by changing the filtering conditions. Furthermore, it provides a powerful way to select multiple optimal target sequences from thousands of genes at once for the creation of a genome-wide library. Cas-Database also provides a web application programming interface (web API) for advanced bioinformatics users.
- URI
- https://academic.oup.com/bioinformatics/article/32/13/2017/1743283https://repository.hanyang.ac.kr/handle/20.500.11754/74644
- ISSN
- 1367-4803; 1460-2059
- DOI
- 10.1093/bioinformatics/btw103
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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