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dc.contributor.author오영석-
dc.date.accessioned2018-08-29T05:26:05Z-
dc.date.available2018-08-29T05:26:05Z-
dc.date.issued2016-07-
dc.identifier.citationBIOLOGY OF REPRODUCTION (2016), v. 95, NO. 1, Page. 5-5en_US
dc.identifier.issn0006-3363-
dc.identifier.issn1529-7268-
dc.identifier.urihttps://academic.oup.com/biolreprod/article/95/1/5,%201-11/2883452-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/74567-
dc.description.abstractTo understand the role of Coxsackievirus and adenovirus receptor (CAR), a tight junction (TJ) protein, in peri-implantation embryos, developmental expression of CAR and its role in paracellular permeability were examined in mouse embryos. Splice variants for transmembrane CAR, Car1, Car2, and Car3 mRNA, were expressed from 2-cell, morula, and blastocyst stages onward, respectively, whereas mRNA for soluble CAR was expressed in MII oocytes and 4-cell stage onward. On Western blot, similar to 46 kDa CAR proteins were detected in blastocysts. During the 4-cell embryos to morula stage, CAR was gradually concentrated at the contacts between blastomeres. In blastocysts, CAR was expressed at the cell contacts within the inner cell mass as well as in the trophectoderm (TE) where CAR was found together with ZO1 at the apical contacts, suggesting that CAR builds up apical TJs in TE and mediates cell adhesion in TE and inner cell mass. In blastocysts, CAR-blocking antibodies under Ca2+ switching increased the dextran permeability and decreased the volume of blastocoel and H19 and Cdx2 mRNA, suggesting the pivotal role of CAR in the blastocyst development and paracellular permeability barrier in TE. CAR was expressed in TE of implanting embryos as well as endometrial epithelium, suggesting the involvement of CAR in the interaction between implanting embryos and endometrium. At 5-6 days postcoitum, CAR was expressed together with ZO1 in the primitive endoderm, visceral endoderm, and epiblasts facing the pro-amniotic cavity, suggesting that CAR TJs contribute to the separation of epiblast from the blastocoel and development of the pro-amniotic cavity within epiblasts.en_US
dc.description.sponsorshipSupported by a grant from the Korea Healthcare Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (A120043).en_US
dc.language.isoenen_US
dc.publisherSOC STUDY REPRODUCTIONen_US
dc.subjectCARen_US
dc.subjectmouseen_US
dc.subjectperi-implantation embryoen_US
dc.subjecttight junctionen_US
dc.titleCoxsackievirus and Adenovirus Receptor, a Tight Junction Protein, in Peri-Implantation Mouse Embryosen_US
dc.typeArticleen_US
dc.relation.no1-
dc.relation.volume95-
dc.identifier.doi10.1095/biolreprod.115.138099-
dc.relation.page5-5-
dc.relation.journalBIOLOGY OF REPRODUCTION-
dc.contributor.googleauthorOh, Yeong Seok-
dc.contributor.googleauthorNah, Won Heum-
dc.contributor.googleauthorChoi, Bomi-
dc.contributor.googleauthorKim, Seok Hyun-
dc.contributor.googleauthorGye, Myung Chan-
dc.relation.code2016001736-
dc.sector.campusS-
dc.sector.daehakRESEARCH INSTITUTE[S]-
dc.sector.departmentTHE RESEARCH INSTITUTE FOR NATURAL SCIENCES-
dc.identifier.pidysoh2001-
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