Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 고현철 | - |
dc.date.accessioned | 2018-08-28T01:01:23Z | - |
dc.date.available | 2018-08-28T01:01:23Z | - |
dc.date.issued | 2016-07 | - |
dc.identifier.citation | BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS, v. 1859, NO 7, Page. 896-905 | en_US |
dc.identifier.issn | 1874-9399 | - |
dc.identifier.issn | 0006-3002 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S1874939915002709 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/74527 | - |
dc.description.abstract | Nonsense-mediated mRNA decay (NMD) modulates the level of mRNA harboring a premature termination codon (PTC) in a translation-dependent manner. Inhibition of translation is known to impair NMD; however, few studies have investigated the correlation between enhanced translation and increased NMD. Here, we demonstrate that insulin signaling events increase translation, leading to an increase in NMD of eIF4E-bound transcripts. We provide evidence that (i) insulin-mediated enhancement of translation augments NMD and rapamycin abrogates this enhancement; (ii) an increase in AKT phosphorylation due to inhibition of PTEN facilitates NMD; (iii) insulin stimulation increases the binding of up-frameshift factor 1 (UPF1), most likely to eIF4E-bound PTC-containing transcripts; and (iv) insulin stimulation induces the colocalization of UPF1 and eIF4E in processing bodies. These results illustrate how extracellular signaling promotes the removal of eIF4E-bound NMD targets. (C) 2015 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | We would like to thank Lynne E. Maquat (University of Rochester) for providing the NMD test and reference plasmids, pACTAG2-HA-4EBP1-TTAA, and anti-UPF1 antibody and Yoon Ki Kim (Korea University) for providing the anti-P-UPF antibody. This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2015R1D1A1A01058878 to J.H.) and by a grant from the Medical Research Center (2008-0062287 to J.H.) funded by the National Research Foundation of the Korea (NRF) of the Ministry of Science, ICT and Future Planning, Republic of Korea. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.subject | PI3K/AKT/mTOR | en_US |
dc.subject | Insulin | en_US |
dc.subject | NMD | en_US |
dc.subject | Translation | en_US |
dc.subject | Processing body | en_US |
dc.title | Insulin Signaling Augments eIF4E-Dependent Nonsense-Mediated mRNA Decay in Mammalian Cells | en_US |
dc.type | Article | en_US |
dc.relation.no | 7 | - |
dc.relation.volume | 1859 | - |
dc.identifier.doi | https://doi.org/10.1016/j.bbagrm.2015.12.006 | - |
dc.relation.page | 896-905 | - |
dc.relation.journal | BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS | - |
dc.contributor.googleauthor | Park, Jungyun | - |
dc.contributor.googleauthor | Ahn, Seyoung | - |
dc.contributor.googleauthor | Jayabalan, Aravinth K.)[ | - |
dc.contributor.googleauthor | Ohn, Takbum | - |
dc.contributor.googleauthor | Koh, Hyun Chul | - |
dc.contributor.googleauthor | Hwang, Jungwook | - |
dc.relation.code | 2016000157 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | hckoh | - |
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