Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤채옥 | - |
dc.date.accessioned | 2018-07-26T02:22:35Z | - |
dc.date.available | 2018-07-26T02:22:35Z | - |
dc.date.issued | 2012-07 | - |
dc.identifier.citation | Gene Therapy, 2012, 19(7), p.711-723 | en_US |
dc.identifier.issn | 0969-7128 | - |
dc.identifier.issn | 1476-5462 | - |
dc.identifier.uri | https://www.nature.com/articles/gt2011125 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/73051 | - |
dc.description.abstract | Interleukin (IL)-12 and granulocyte-monocyte colony-stimulating factor (GM-CSF) have recently been used as immunotherapeutic agents in cancer gene therapy. IL-12 and GM-CSF have differential roles in the antitumor immune response, as IL-12 targets T, NK and natural killer T (NKT) cells and GM-CSF principally targets antigen-presenting cells (APCs). To strengthen the therapeutic efficacy of these two cytokines, we generated an oncolytic adenovirus (Ad), Ad-ΔB7/IL12/GMCSF, coexpressing IL-12 and GM-CSF. Using a murine B16-F10 syngeneic tumor model, we show that Ad-ΔB7/IL12/GMCSF promoted antitumor responses and increased survival compared with an oncolytic Ad expressing IL-12 or GM-CSF alone (Ad-ΔB7/IL12 or Ad-ΔB7/GMCSF, respectively). By measuring cytotoxic T lymphocyte activity and interferon-γ production, we show that the enhanced therapeutic effect was mediated by the induction of immune cell cytotoxicity. In situ delivery of Ad-ΔB7/IL12/GMCSF resulted in massive infiltration of CD4+ T cells, CD8+ T cells, NK cells and CD86+ APCs into the tissue surrounding the necrotic area of the tumor. Moreover, GM-CSF effectively promoted antitumor immune memory, which was significantly augmented by IL-12. Lastly, IL12-expressing oncolytic Ads prevented tumor-induced thymic atrophy and was associated with reduced apoptosis and increased proliferation in the thymus. Taken together, these data demonstrate that an oncolytic Ad coexpressing IL-12 and GM-CSF is a potential therapeutic tool for the treatment of cancer. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.title | Strengthening of antitumor immune memory and prevention of thymic atrophy mediated by adenovirus expressing IL-12 and GM-CSF | en_US |
dc.type | Article | en_US |
dc.relation.no | 7 | - |
dc.relation.volume | 19 | - |
dc.identifier.doi | 10.1038/gt.2011.125 | - |
dc.relation.page | 711-723 | - |
dc.relation.journal | GENE THERAPY | - |
dc.contributor.googleauthor | Choi, K. J. | - |
dc.contributor.googleauthor | Zhang, S. N. | - |
dc.contributor.googleauthor | Choi, I. K. | - |
dc.contributor.googleauthor | Kim, J. S. | - |
dc.contributor.googleauthor | Yun, C. O. | - |
dc.relation.code | 2012203375 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | chaeok | - |
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