Creation of Stepwise Concentration Gradient in Picoliter Droplets for Parallel Reactions of Matrix Metalloproteinase II and IX
- Title
- Creation of Stepwise Concentration Gradient in Picoliter Droplets for Parallel Reactions of Matrix Metalloproteinase II and IX
- Author
- Jong Wook Hong
- Issue Date
- 2011-04
- Publisher
- AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
- Citation
- ANALYTICAL CHEMISTRY,권: 83 호: 9 페이지: 3358-3364
- Abstract
- We present a new methodology for generating a stepwise concentration gradient in a series of microdroplets by using monolithic micro valves that act as "faucets" in micrometer-scale. A distinct concentration gradient of a substrate was generated for the determination of the kinetic parameters of two different enzymes using only 10 picoliter-scale droplets. With a single experiment on a chip, we obtained K(M) and k(cat) values of matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9), and compared the catalytic competence of the two enzymes. The present system and method are highly suitable for applications where the reagents or samples are limited and precious.
- URI
- https://pubs.acs.org/doi/abs/10.1021/ac103217phttps://repository.hanyang.ac.kr/handle/20.500.11754/72807
- ISSN
- 0003-2700
- DOI
- 10.1021/ac103217p
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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