Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박현희 | - |
dc.date.accessioned | 2018-07-24T08:21:46Z | - |
dc.date.available | 2018-07-24T08:21:46Z | - |
dc.date.issued | 2011-01 | - |
dc.identifier.citation | Brain Research, 2011, 1370, P.53-63 | en_US |
dc.identifier.issn | 0006-8993 | - |
dc.identifier.issn | 1872-6240 | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0006899310025345?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/72754 | - |
dc.description.abstract | Neurotoxic effects have been suggested for L-3,4-dihydroxyphenylalanine (L-DOPA), while neuroprotective effects have been proposed for statins. We investigated whether certain statins (simvastatin or pitavastatin) could inhibit L-DOPA neurotoxicity. Neuronally-differentiated PC12 (nPC12) cells were treated with L-DOPA and/or statins for 24 h, and their viabilities were measured using a cell counting kit, trypan blue staining, and 4',6-diamidino-2-phenylindole (DAPI) staining. Free radical and specific intracellular signaling protein levels were measured with 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) and Western blotting, respectively. High concentrations of L-DOPA reduced nPC12 cell viability, but combined treatment with statins restored viability. Treatment with 200 mu M L-DOPA increased free radical and hydroxyl radical levels, but combined treatment with 5 mu M statins decreased these levels. Survival-related signaling proteins were decreased in nPC12 cells treated with 200 mu M L-DOPA, but combined treatment with 5 mu M statins significantly increased the levels of these proteins. Treatment with 200 mu M L-DOPA significantly increased death-related signaling proteins, while combined treatment with 5 mu M statins reduced the levels of these proteins. Pretreatment with LY294002, a phosphatidylinositol 3 kinase (PI3K) inhibitor, before combined treatment with statins and L-DOPA almost completely blocked the protective effects of statins. These results indicate that statins reduce L-DOPA neurotoxicity by lowering oxidative stress and by enhancing survival signals and inhibiting death signals via activation of the PI3K pathway. | en_US |
dc.description.sponsorship | This work was supported by a grant from the Research Fund of Hanyang University (HY-2010-MC). | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Science B.V | en_US |
dc.subject | L-DOPA | en_US |
dc.subject | Statin | en_US |
dc.subject | Phosphatidylinositol 3-kinase | en_US |
dc.subject | Neurotoxicity | en_US |
dc.title | Protective effects of statins on L-DOPA neurotoxicity due to the activation of phosphatidylinositol 3-kinase and free radical scavenging in PC12 cell culture | en_US |
dc.type | Article | en_US |
dc.relation.volume | 1370 | - |
dc.identifier.doi | 10.1016/j.brainres.2010.11.021 | - |
dc.relation.page | 53-63 | - |
dc.relation.journal | BRAIN RESEARCH | - |
dc.contributor.googleauthor | Koh, Seong-Ho | - |
dc.contributor.googleauthor | Park, Hyun-Hee | - |
dc.contributor.googleauthor | Choi, Na-Young | - |
dc.contributor.googleauthor | Lee, Kyu-Yong | - |
dc.contributor.googleauthor | Kim, Sangjae | - |
dc.contributor.googleauthor | Lee, Young Joo | - |
dc.contributor.googleauthor | Kim, Hee-Tae | - |
dc.relation.code | 2011201421 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | newdiaz | - |
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