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Microfluidic Biochips for Simple Impedimetric Detection of Thrombin Based on Label-free DNA Aptamers

Title
Microfluidic Biochips for Simple Impedimetric Detection of Thrombin Based on Label-free DNA Aptamers
Author
안유민
Keywords
DNA Aptamer; Microfluidics; Thrombin; Electrochemical impedance spectroscopy; Anti-thrombin aptamer; POINT-OF-CARE; ELECTROCHEMICAL BIOSENSORS; GOLD NANOPARTICLES; DIAGNOSTICS; PROTEIN; IMMUNOASSAY; ENHANCEMENT; SEPARATION; SYSTEM; ASSAYS
Issue Date
2017-06
Publisher
KOREAN BIOCHIP SOCIETY-KBCS
Citation
BIOCHIP JOURNAL, v. 11, No. 2, Page. 109-115
Abstract
This paper presents a proof-of-concept microfluidic aptamer-based sensor for thrombin point of -care testing using electrochemical impedance spectroscopy. The disposable biosensor is composed of a polydimethylsiloxane (PDMS) channel layer over a glass substrate. The substrate surface has an Au working electrode and a Pt reference/counter electrode. In this study, human thrombin was used as a biomarker for disease diagnosis. An unlabeled aptamer specific to thrombin was immobilized on a working electrode and electrochemical impedance was measured as thrombin was injected into the biosensor. Thrombin was detected by measuring change in impedance. The proposed biochip had a detection range of 0.1-100,000 ng mL(-1) for thrombin concentration and a limit of detection of 0.1 ng mL(-1). The results indicated that our biochip could be an effective tool for other molecular diagnostic systems.
URI
https://link.springer.com/article/10.1007/s13206-016-1203-7https://repository.hanyang.ac.kr/handle/20.500.11754/72155
ISSN
1976-0280; 2092-7843
DOI
10.1007/s13206-016-1203-7
Appears in Collections:
COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > MECHANICAL ENGINEERING(기계공학과) > Articles
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