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Spontaneously Differentiated GATA6-Positive Human Embryonic Stem Cells Represent an Important Cellular Step in Human Embryonic Development

Title
Spontaneously Differentiated GATA6-Positive Human Embryonic Stem Cells Represent an Important Cellular Step in Human Embryonic Development
Author
김계성
Keywords
Biomarkers; metabolism; Bone Morphogenetic Protein 2; genetics; Bone Morphogenetic Protein 4; Carrier Proteins; pharmacology; Cell Differentiation; Cell Line; Embryonic Development; Embryonic Stem Cells; cytology; drug effects; Endoderm; GATA6 Transcription Factor; Gene Expression Regulation; Developmental; Homeodomain Proteins; Humans; Octamer Transcription Factor-3; Prealbumin
Issue Date
2013-10
Publisher
MARY ANN LEIBERT
Citation
Stem Cells and Development, 2013, 22(20), P.2706-2719
Abstract
In this study, we isolated and characterized spontaneously differentiated human embryonic stem cells (SD-hESCs) found in hESC colonies in comparison to the morphologically premature ESCs in the colonies to investigate the potential role of SD-hESCs in embryogenesis. SD-hESCs were distinguished from undifferentiated hESCs by their higher expression of GATA6, a marker for primitive endoderm and transthyretin, a marker visceral endoderm in embryoid bodies (EBs). SD-hESCs expressed OCT4 and NANOG, markers for pluripotent stem cells, at significantly lower levels than undifferentiated hESCs. EBs derived from isolated SD-hESCs were morphologically distinct from cells directly derived from the undifferentiated hESCs; they contained higher number of cysts compared to EBs from undifferentiated hESC-derived EBs (42% vs. 20%). Furthermore, the extracellular signal molecule, BMP2/4, induced a higher GATA4/6 expression and cystic EB formation than control and noggin-treated EBs. Since cystic formation in EBs play a role in primitive endoderm formation during embryogenesis, the SD-hESC may be a relevant cell type equipped to differentiate into primitive endoderm. Our results suggest that SD-ESCs generated during routine hESC culture are not just an artifact of in vitro culture and these cells could serve as a useful model to study the process of embryogenesis.
URI
https://www.liebertpub.com/doi/abs/10.1089/scd.2013.0083https://repository.hanyang.ac.kr/handle/20.500.11754/70820
ISSN
1547-3287; 1557-8534
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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