Human aortic endothelial cells compare favourably with macrophages for the study of anthrax toxins
- Title
- Human aortic endothelial cells compare favourably with macrophages for the study of anthrax toxins
- Author
- 김성민
- Keywords
- anthrax toxins; protective antigen; lethal toxin; edema toxin; macrophages; human aortic endothelial cells
- Issue Date
- 2013-07
- Publisher
- Inderscience Enterprises Ltd
- Citation
- INTERNATIONAL JOURNAL OF NANOTECHNOLOGY, 권: 10, 호: 8-9, 페이지: 756-770
- Abstract
- Anthrax is an infectious disease caused by Bacillus anthracis. Although B. anthracis is rare in the natural environment, anthrax spores can be produced in vitro and potentially used as a biological weapon. Thus, understanding the molecular pathogenesis of anthrax is a critical concern for national security. The aim of this study was to compare the effects of anthrax toxins, lethal toxin (LeTx), and edema toxin (EdTx) on human aortic endothelial cells (HAECs) and the J774A. 1 murine macrophage cell line. We analysed cell viability, adhesion, morphology, and lipopolysaccharide (LPS)-stimulated cytokine production after incubation of cells with varying concentrations of the toxins. Both LeTx and EdTx markedly inhibited LPS-induced transcription of tumour necrosis factor alpha (TNF-alpha), interleukin (IL)-1 alpha, and IL-6 in J774A. 1 cells. In contrast, EdTx synergised with LPS to increase the transcription of IL-6 and IL-8 in HAECs. We showed that HAECs are suitable for anthrax toxin research and express higher levels of the two anthrax toxin receptors - tumour endothelial marker 8 (TEM8/ANTXR1) and capillary morphogenesis protein 2 (CMG2/ANTXR2) - than do J774A. 1 cells. Collectively, our results suggest that HAECs may be superior to macrophages for the study of anthrax pathogenesis.
- URI
- https://www.researchgate.net/profile/Nirmal_Rajasekaran2/publication/250614935_Human_aortic_endothelial_cells_compare_favourably_with_macrophages_for_the_study_of_anthrax_toxins/links/55470cca0cf24107d3980ebe.pdfhttps://repository.hanyang.ac.kr/handle/20.500.11754/70742
- ISSN
- 1475-7435
- DOI
- 10.1504/IJNT.2013.054216
- Appears in Collections:
- COLLEGE OF ART AND PHYSICAL EDUCATION[S](예술·체육대학) > PHYSICAL EDUCATION(체육학과) > Articles
- Files in This Item:
- IJNT108-9Paper14.pdfDownload
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