Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 임동우 | - |
dc.date.accessioned | 2018-04-19T09:14:20Z | - |
dc.date.available | 2018-04-19T09:14:20Z | - |
dc.date.issued | 2012-03 | - |
dc.identifier.citation | Biochip Journal, 2012, 6(1), P.10-16 | en_US |
dc.identifier.issn | 1976-0280 | - |
dc.identifier.uri | http://link.springer.com/article/10.1007%2Fs13206-012-6102-y | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/69578 | - |
dc.description.abstract | In this work, we report on a simple process for fabricating a hydrophobic/hydrophilic hybrid-patterned microarray chip for a fast and sensitive immunoassay. Two different types of self-assembled monolayers (SAMs) were used in the fabrication of hydrophilic well patterns and hydrophobic substrates. The hydrophilic/hydrophobic hybrid SAM pattern generates a clear-cut boundary between the sample and the background. A change in the precursor molecules allows for many different. types of SAMs to be employed in the fabrication process. Fluorescence image-based detection has previously been used for the quantitative immune-analysis of a specific cancer marker. Here, a titanium-coated glass substrate was utilized to suppress auto-fluorescence signals from substrate backgrounds. Angiogenin (ANG), a small polypeptide implicated in both angiogenesis and tumor growth, was used as a target cancer marker for its validation. Assay results demonstrate that the hybrid-patterned array chip yields a narrower error deviation and a lower coefficient variation than in a conventional 96-well plate ELISA. Furthermore, the sample requirement (1 mu L) for the hybrid-patterned chip is about 50 times less than that required in an ELISA (at least 50 mu L). The proposed hydrophobic/hydrophilic hybrid-patterned microarray chip is expected to be a highly efficient tool that can be applied to a high throughput immunoassay of a specific cancer marker. | en_US |
dc.description.sponsorship | This work was supported by the National Research Foundation of Korea (grant numbers R11-2010-044-1002-0, 2010K001236 and K20904000004 -10A0500-00410). This work was also partially supported by the Ministry of Knowledge Economy (MKE) and the Korea Industrial Technology Foundation (KOTEF) through the Human Resource Training Project for Strategic Technology. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Korean Biochip Society | en_US |
dc.subject | Hydrophobic/hydrophilic pattern | en_US |
dc.subject | SAM | en_US |
dc.subject | Microarray | en_US |
dc.subject | Immunoassay | en_US |
dc.subject | Angiogenin | en_US |
dc.title | Fabrication of a hydrophobic/hydrophilic hybrid-patterned microarray chip and its application to a cancer marker immunoassay | en_US |
dc.title.alternative | hydrophilic hybrid-patterned microarray chip and its application to a cancer marker immunoassay | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 6 | - |
dc.identifier.doi | 10.1007/s13206-012-6102-y | - |
dc.relation.page | 10-16 | - |
dc.relation.journal | BIOCHIP JOURNAL | - |
dc.contributor.googleauthor | Lee, Moonkwon | - |
dc.contributor.googleauthor | Kim, Ki Hyung | - |
dc.contributor.googleauthor | Park, Jin-Goo | - |
dc.contributor.googleauthor | Lee, Jung-Hwan | - |
dc.contributor.googleauthor | Lim, Hyun-Woo | - |
dc.contributor.googleauthor | Park, Min-Yi | - |
dc.contributor.googleauthor | Chang, Soo-Ik | - |
dc.contributor.googleauthor | Lee, Eun Kyu | - |
dc.contributor.googleauthor | Lim, Dong Woo | - |
dc.contributor.googleauthor | Choo, Jaebum | - |
dc.relation.code | 2012217720 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | dlim | - |
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