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dc.contributor.advisor김용희-
dc.contributor.author천연지-
dc.date.accessioned2018-04-18T06:07:01Z-
dc.date.available2018-04-18T06:07:01Z-
dc.date.issued2018-02-
dc.identifier.urihttp://www.dcollection.net/handler/hanyang/000000104846en_US
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/68175-
dc.description.abstractIt is a study on development of bio-inspired poly (lactic-co-glycolic acid) (PLGA) microparticles. Although PLGA have proved several advances as a biomaterial, a few drawbacks are still present. For therapeutic effect, the PLGA particles should cross an endothelial layer as drug carriers. I got inspired from CD14+ monocytes, inflammation related human monocytes, that migrates the endothelial layer under inflamed condition. I try to mimic the endothelial layer crossing ability by cloaking the CD14+ monocytes membranes on PLGA microparticles. Preparation of the PLGA microparticles was conducted by emulsion method using dichloromethane. CD14+ Monocytes membranes are cloaked to PLGA microparticles by sonication after going through several freeze-thaw cycles. Uniform size distribution and surface morphology was also confirmed by Transmission Electron Microscope and Scanning Electron Microscope. As the CD14 is a marker for the human monocyte, the PLGA microparticles with CD14+ monocytes membranes were visualized by confocal microscope using FITC-labeled anti-CD14 Antibody. Compared to uncoated PLGA microparticles, the PLGA microparticles with CD14+ monocytes membranes showed increased cellular uptake. Also enhanced endothelial cell layer permeability of PLGA microparticles with CD14+ monocytes membranes was observed through transwell study. In addition, substantiation of experimental result using human monocytes proposes the “node” to clinical trial for inflammatory disease.-
dc.publisher한양대학교-
dc.title생체모방형 마이크로입자 개발-
dc.title.alternativeDevelopment of bio-inspired poly(lactic-co-glycolic acid) microparticles-
dc.typeTheses-
dc.contributor.googleauthor천연지-
dc.sector.campusS-
dc.sector.daehak대학원-
dc.sector.department생명공학과-
dc.description.degreeMaster-
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GRADUATE SCHOOL[S](대학원) > BIOENGINEERING(생명공학과) > Theses (Master)
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