It is a study on development of bio-inspired poly (lactic-co-glycolic acid) (PLGA) microparticles. Although PLGA have proved several advances as a biomaterial, a few drawbacks are still present. For therapeutic effect, the PLGA particles should cross an endothelial layer as drug carriers. I got inspired from CD14+ monocytes, inflammation related human monocytes, that migrates the endothelial layer under inflamed condition. I try to mimic the endothelial layer crossing ability by cloaking the CD14+ monocytes membranes on PLGA microparticles. Preparation of the PLGA microparticles was conducted by emulsion method using dichloromethane. CD14+ Monocytes membranes are cloaked to PLGA microparticles by sonication after going through several freeze-thaw cycles. Uniform size distribution and surface morphology was also confirmed by Transmission Electron Microscope and Scanning Electron Microscope. As the CD14 is a marker for the human monocyte, the PLGA microparticles with CD14+ monocytes membranes were visualized by confocal microscope using FITC-labeled anti-CD14 Antibody. Compared to uncoated PLGA microparticles, the PLGA microparticles with CD14+ monocytes membranes showed increased cellular uptake. Also enhanced endothelial cell layer permeability of PLGA microparticles with CD14+ monocytes membranes was observed through transwell study. In addition, substantiation of experimental result using human monocytes proposes the “node” to clinical trial for inflammatory disease.