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dc.contributor.author이영복-
dc.date.accessioned2018-04-18T05:50:59Z-
dc.date.available2018-04-18T05:50:59Z-
dc.date.issued2016-05-
dc.identifier.citationBULLETIN OF THE KOREAN CHEMICAL SOCIETY, v. 37, NO 6, Page. 810-814en_US
dc.identifier.issn0253-2964-
dc.identifier.issn1229-5949-
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/abs/10.1002/bkcs.10773-
dc.identifier.urihttps://repository.hanyang.ac.kr/handle/20.500.11754/68086-
dc.description.abstractAffording several orders of signal enhancement compared to conventional nuclear magnetic resonance (NMR), dynamic nuclear polarization (DNP) enables direct observation of the most common C-13 nuclei in organic chemistry and biochemistry at low concentrations. These nuclei have poor sensitivity due to low natural abundance and a low gyromagnetic ratio. Herein, we report an application of this technique to a multi-step enzymatic reaction. The main aim of this study was to demontrate the effect of deuteration on specific spins of interest for the real-time trypsin hydrolysis reaction of synthesized N-alpha-benzoyl-L-arginine methyl-d(3) ester (BAME-d(3)). Kinetic parameter and fit accuracy were analyzed in order to determine the influence of deuteration.en_US
dc.description.sponsorshipThis work was supported by the research fund of Hanyang University (HY-2014-N).en_US
dc.language.isoenen_US
dc.publisherWILEY-V C H VERLAG GMBHen_US
dc.subjectHyperpolarizationen_US
dc.subjectNuclear magnetic resonance spectroscopyen_US
dc.subjectEnzyme kineticsen_US
dc.subjectDeuterationen_US
dc.subjectReaction intermediatesen_US
dc.titleHyperpolarized NMR Analysis of Enzymatic Reaction: Extension of Observable Reaction Time by Deuterium Isotope Labelingen_US
dc.typeArticleen_US
dc.relation.no6-
dc.relation.volume37-
dc.identifier.doi10.1002/bkcs.10773-
dc.relation.page810-814-
dc.relation.journalBULLETIN OF THE KOREAN CHEMICAL SOCIETY-
dc.contributor.googleauthorLee, Youngbok-
dc.relation.code2016002474-
dc.sector.campusS-
dc.sector.daehakGRADUATE SCHOOL[S]-
dc.sector.departmentDEPARTMENT OF BIONANOTECHNOLOGY-
dc.identifier.pidyblee-
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GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Articles
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