Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤채옥 | - |
dc.date.accessioned | 2018-04-16T02:51:57Z | - |
dc.date.available | 2018-04-16T02:51:57Z | - |
dc.date.issued | 2012-10 | - |
dc.identifier.citation | Gene Therapy, Jul 2012, 19(7), p711-723. 13p. | en_US |
dc.identifier.issn | 0969-7128 | - |
dc.identifier.uri | https://www.nature.com/articles/gt2011125 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/67610 | - |
dc.description.abstract | Interleukin (IL)-12 and granulocyte-monocyte colony-stimulating factor (GM-CSF) have recently been used as immunotherapeutic agents in cancer gene therapy. IL-12 and GM-CSF have differential roles in the antitumor immune response, as IL-12 targets T, NK and natural killer T (NKT) cells and GM-CSF principally targets antigen-presenting cells (APCs). To strengthen the therapeutic efficacy of these two cytokines, we generated an oncolytic adenovirus (Ad), Ad-Delta B7/IL12/GMCSF, coexpressing IL-12 and GM-CSF. Using a murine B16-F10 syngeneic tumor model, we show that Ad-Delta B7/IL12/GMCSF promoted antitumor responses and increased survival compared with an oncolytic Ad expressing IL-12 or GM-CSF alone (Ad-Delta B7/IL12 or Ad-Delta B7/GMCSF, respectively). By measuring cytotoxic T lymphocyte activity and interferon-gamma production, we show that the enhanced therapeutic effect was mediated by the induction of immune cell cytotoxicity. In situ delivery of Ad-Delta B7/IL12/GMCSF resulted in massive infiltration of CD4(+) T cells, CD8(+) T cells, NK cells and CD86(+) APCs into the tissue surrounding the necrotic area of the tumor. Moreover, GM-CSF effectively promoted antitumor immune memory, which was significantly augmented by IL-12. Lastly, IL12-expressing oncolytic Ads prevented tumor-induced thymic atrophy and was associated with reduced apoptosis and increased proliferation in the thymus. Taken together, these data demonstrate that an oncolytic Ad coexpressing IL-12 and GM-CSF is a potential therapeutic tool for the treatment of cancer. Gene Therapy (2012) 19, 711-723; doi:10.1038/gt.2011.125; published online 13 October 2011 | en_US |
dc.description.sponsorship | This work was supported by Grants from the Ministry of Knowledge Economy (10030051, Dr C-O Yun), the Korea Science and Engineering Foundation (R15-2004-024-02001-0, 2009K001644, 2010-0029220, Dr C-O. Yun), the Korea Food and Drug Administration (KFDA-10172-332 to Dr C-O Yun), and Yonsei University College of Medicine faculty research Grant (6-2010-0052, Dr C-O Yun). Kyung-Ju Choi, Song-Nan Zhang and Ji-Seong Kim are graduate students sponsored by the Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea. Il-Kyu Choi is a graduate student sponsored by KOSEF through National Core Research Center for Nanomedical Technology, Seoul, South Korea. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.subject | IL-12 | en_US |
dc.subject | GM-CSF | en_US |
dc.subject | oncolytic adenovirus | en_US |
dc.subject | RECURRENT PROSTATE-CANCER | en_US |
dc.subject | ONCOLYTIC ADENOVIRUS | en_US |
dc.subject | GENE-THERAPY | en_US |
dc.subject | TUMOR-GROWTH | en_US |
dc.subject | PHASE-I | en_US |
dc.subject | THYMOCYTE PROLIFERATION | en_US |
dc.subject | REPLICATION-COMPETENT | en_US |
dc.subject | DENDRITIC CELLS | en_US |
dc.subject | MURINE MAMMARY | en_US |
dc.subject | BREAST-CANCER | en_US |
dc.title | Strengthening of antitumor immune memory and prevention of thymic atrophy mediated by adenovirus expressing IL-12 and GM-CSF | en_US |
dc.type | Article | en_US |
dc.relation.no | 7 | - |
dc.relation.volume | 19 | - |
dc.identifier.doi | 10.1038/gt.2011.125 | - |
dc.relation.page | 711-723 | - |
dc.relation.journal | CANCER GENE THERAPY | - |
dc.contributor.googleauthor | Choi, K. J. | - |
dc.contributor.googleauthor | Zhang, S. N. | - |
dc.contributor.googleauthor | Choi, I. K. | - |
dc.contributor.googleauthor | Kim, J. S. | - |
dc.contributor.googleauthor | Yun, C. O. | - |
dc.relation.code | 2012201679 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | chaeok | - |
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