Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이양순 | - |
dc.date.accessioned | 2018-04-16T02:22:16Z | - |
dc.date.available | 2018-04-16T02:22:16Z | - |
dc.date.issued | 2012-02 | - |
dc.identifier.citation | JOURNAL OF MEDICAL MICROBIOLOGY, 2012, 61(2), P.274p ~ 281 | en_US |
dc.identifier.issn | 0022-2615 | - |
dc.identifier.uri | http://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.035618-0 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/67502 | - |
dc.description.abstract | Toxigenic Clostridium difficile culture is considered to be the standard diagnostic method for the detection of C. difficile infection (CDI). Culture methods are time-consuming and although enzyme immunoassay is rapid and easy to use, it has low sensitivity. In the present study, the AdvanSure CD real-time (RT)-PCR kit (LG Life Sciences) was evaluated for its ability to detect C. difficile toxin A (tcdA) and B (tcdB) genes, simultaneously. A total of 127 fresh diarrhoeal stool specimens, submitted to the clinical microbiology laboratory for C. difficile culture, were tested. C. difficile toxins and toxin genes were detected with a VIDAS C. difficile A&B (VIDAS-CDAB) enzyme-linked fluorescent immunoassay (ELFA) and the AdvanSure RT-PCR kit, respectively, according to the manufacturers’ instructions. Their performance was compared with a standard toxigenic culture method as a reference. The sensitivity, specificity and positive and negative predictive values using the AdvanSure RT-PCR kit were 100 %, 98.3 %, 84.6 % and 100 %, respectively, while those of the VIDAS-CDAB system were 63.6 %, 100 %, 100 % and 96.6 %, respectively. Four tcdA +/tcdB + strains of C. difficile were detected with the AdvanSure RT-PCR kit, which offers comparable sensitivity and specificity to the reference method with a turnaround time of ~3 hours. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.subject | Bacterial Proteins | en_US |
dc.subject | analysis | en_US |
dc.subject | genetics | en_US |
dc.subject | Bacterial Toxins | en_US |
dc.subject | Bacteriological Techniques | en_US |
dc.subject | methods | en_US |
dc.subject | Clostridium Infections | en_US |
dc.subject | diagnosis, microbiology | en_US |
dc.subject | Clostridium difficile | en_US |
dc.subject | isolation & purification | en_US |
dc.subject | Enterotoxins | en_US |
dc.subject | Feces | en_US |
dc.title | Detection of Clostridium difficile toxin A/B genes by multiplex real-time PCR for the diagnosis of C. difficile infection | en_US |
dc.title.alternative | B genes by multiplex real-time PCR for the diagnosis of C. difficile infection | en_US |
dc.type | Article | en_US |
dc.relation.volume | 61 | - |
dc.identifier.doi | 10.1099/jmm.0.035618-0 | - |
dc.relation.page | 274-277 | - |
dc.relation.journal | JOURNAL OF MEDICAL MICROBIOLOGY | - |
dc.contributor.googleauthor | Heejung, Kim | - |
dc.contributor.googleauthor | Seok Hoon, Jeong | - |
dc.contributor.googleauthor | Myungsook, Kim | - |
dc.contributor.googleauthor | Yangsoon, Lee | - |
dc.contributor.googleauthor | Kyungwon, Lee | - |
dc.relation.code | 2012205407 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | yangsoon | - |
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