Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2018-04-16T02:13:46Z | - |
dc.date.available | 2018-04-16T02:13:46Z | - |
dc.date.issued | 2012-02 | - |
dc.identifier.citation | Journal of Controlled Release, Vol.158, No.1 [2012], p131-138 | en_US |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0168365911010315 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/67453 | - |
dc.description.abstract | The R3V6 peptides, which are composed of a 3-arginine block and a 6-valine block, formed self-assembled micelles in aqueous solution. Dye quenching assays showed that a hydrophobic fluorescent dye, 5-dodecanoylaminofluorescein (DAF), interacted with and was loaded into the hydrophobic core of the micelles. In this study, dexamethasone-loaded R3V6 peptide micelles (R3V6-Dexa) were evaluated as a gene carrier. R3V6-Dexa had higher gene delivery efficiency in human embryonic kidney 293 cells compared to those of the R3V6 peptides and poly-L-lysine (PLL). Dexamethasone might stabilize the micelle structure of the R3V6 peptides by forming strong hydrophobic cores and enhanced the transfection efficiency. Furthermore, R3V6-Dexa reduced the expression of an inflammatory cytokine, interleukin-6 (IL-6), more efficiently in lipopolysaccharide (LPS)-induced Raw264.7 cells than did dexamethasone, suggesting that R3V6-Dexa is also a useful carrier for dexamethasone delivery. A focal brain ischemia-reperfusion model was produced by middle cerebral artery occlusion (MCAO). A heme oxygenase-1 (HO-1) expression plasmid DNA, pSV-HO-1, was delivered into the brain using R3V6-Dexa as a carrier. The pSV-HO-1/R3V6-Dexa complex was injected into the brain 1 hr prior to MCAO. Twenty-four hours later, the HO-1 expression of the pSV-HO-1/R3V6-Dexa injection group was higher than those of the MCAO control, p beta-Luc/R3V6-Dexa, and pSV-HO-1/PEI25k injection groups. In addition, the infarct size was reduced due to the delivery of pSV-HO-1/R3V6-Dexa complex. Therefore, R3V6-Dexa may be a useful carrier for HO-1 gene delivery and stroke gene therapy. | en_US |
dc.description.sponsorship | This work was supported by funding from the Ministry of Education, Science and Technology in Korea (2011K000803 and 20110027482) and by the KORUS Tech Program funded by the Ministry of Knowledge Economy in Korea (Grant no. KT-2008-NT-APFS0-0001). | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Science B.V., Amsterdam. | en_US |
dc.subject | Gene delivery | en_US |
dc.subject | Gene therapy | en_US |
dc.subject | Peptide | en_US |
dc.subject | Micelle | en_US |
dc.subject | Stroke | en_US |
dc.title | Dexamethasone-loaded peptide micelles for delivery of the heme oxygenase-1 gene to ischemic brain | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 158 | - |
dc.identifier.doi | 10.1016/j.jconrel.2011.11.001 | - |
dc.relation.page | 131-138 | - |
dc.relation.journal | JOURNAL OF CONTROLLED RELEASE | - |
dc.contributor.googleauthor | Lee, J. | - |
dc.contributor.googleauthor | Hyun, H. | - |
dc.contributor.googleauthor | Kim, J. | - |
dc.contributor.googleauthor | Ryu, J. H. | - |
dc.contributor.googleauthor | Kim, H. A. | - |
dc.contributor.googleauthor | Park, J. H. | - |
dc.contributor.googleauthor | Lee, M. | - |
dc.relation.code | 2012204927 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
dc.identifier.researcherID | 7409120793 | - |
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