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Stabilization of RNT-1 Protein, Runt-related Transcription Factor (RUNX) Protein Homolog of Caenorhabditis elegans, by Oxidative Stress through Mitogen-activated Protein Kinase Pathway

Title
Stabilization of RNT-1 Protein, Runt-related Transcription Factor (RUNX) Protein Homolog of Caenorhabditis elegans, by Oxidative Stress through Mitogen-activated Protein Kinase Pathway
Author
심지원
Keywords
C-ELEGANS; DEPENDENT PHOSPHORYLATION; MEDIATED DEGRADATION; CELL-PROLIFERATION; MAPK PHOSPHATASE; OSTEOCALCIN GENE; EXPRESSION; METHYLATION; RECEPTOR; CANCER
Issue Date
2012-03
Publisher
Amer SOC Biochemistry
Citation
Journal of Biological Chemistry, 2012, 287(13), P.10444-10452
Abstract
RUNX proteins are evolutionarily conserved transcription factors known to be involved in various developmental processes. Here we report a new role for a RUNX protein: a role in stress response. We show that RNT-1, the Caenorhabditis elegans RUNX homolog, is constantly produced and degraded by the ubiquitination-proteasome pathway in the intestine of the nematode. RNT-1 was rapidly stabilized by oxidative stress, and the rnt-1-mutant animals were more sensitive to oxidative stress, indicating that rapid RNT-1 stabilization is a defense response against the oxidative stress. The MAP kinase pathway is required for RNT-1 stabilization, and RNT-1 was phosphorylated by SEK-1/PMK-1 in vitro. ChIP-sequencing analysis revealed a feedback loop mechanism of the MAP kinase pathway by the VHP-1 phosphatase in the RNT-1-mediated oxidative stress response. We propose that rnt-1 is regulated at the protein level for its role in the immediate response to environmental challenges in the intestine.
URI
http://www.jbc.org/content/287/13/10444http://hdl.handle.net/20.500.11754/67420
ISSN
0021-9258; 1083-351X
DOI
10.1074/jbc.M111.314146
Appears in Collections:
COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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