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Design and analysis of a cross-type structured-illumination confocal microscope for high speed and high resolution

Title
Design and analysis of a cross-type structured-illumination confocal microscope for high speed and high resolution
Author
유홍기
Keywords
confocal; structured illumination; cross-type illumination; high speed
Issue Date
2012-10
Publisher
IOP PUBLISHING LTD
Citation
MEASUREMENT SCIENCE AND TECHNOLOGY, OCT 2012, 23(10), 7p.
Abstract
There have been many studies about a super resolution microscope for many years. A super resolution microscope can detect the physical phenomena or morphology of a biological sample more precisely than conventional microscopes. The structured-illumination microscope (SIM) is one of the technologies that demonstrate super resolution. However, the conventional SIM requires more time to obtain one resolution-enhanced image than other super resolution microscopes. More specifically, the conventional SIM uses three images with a 120° phase difference for each direction and three different directions are image-processed to make one resolution enhancement by increasing the optical transfer function in three directions. In this paper, we present a novel cross structured-illumination confocal microscope (CSICM) that takes the advantage of the technology of both SIM and the confocal microscope. The CSICM uses only two directions with three phase difference images, for a total of six images. By reducing the number of images that must be obtained, the total image acquisition time and image reconstruction time in obtaining the final output images can be decreased, and the confocal microscope provides axial information of the sample automatically. We demonstrate our method of cross illumination and evaluate the performance of the CSICM and compare it to the conventional SIM and the confocal microscope.
URI
http://iopscience.iop.org/article/10.1088/0957-0233/23/10/105403/metahttp://hdl.handle.net/20.500.11754/67318
ISSN
0957-0233
DOI
10.1088/0957-0233/23/10/105403
Appears in Collections:
COLLEGE OF ENGINEERING[S](공과대학) > ELECTRICAL AND BIOMEDICAL ENGINEERING(전기·생체공학부) > Articles
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