Design and analysis of a cross-type structured-illumination confocal microscope for high speed and high resolution
- Title
- Design and analysis of a cross-type structured-illumination confocal microscope for high speed and high resolution
- Author
- 유홍기
- Keywords
- confocal; structured illumination; cross-type illumination; high speed
- Issue Date
- 2012-10
- Publisher
- IOP PUBLISHING LTD
- Citation
- MEASUREMENT SCIENCE AND TECHNOLOGY, OCT 2012, 23(10), 7p.
- Abstract
- There have been many studies about a super resolution microscope for many years. A super resolution microscope can detect the physical phenomena or morphology of a biological sample more precisely than conventional microscopes. The structured-illumination microscope (SIM) is one of the technologies that demonstrate super resolution. However, the conventional SIM requires more time to obtain one resolution-enhanced image than other super resolution microscopes. More specifically, the conventional SIM uses three images with a 120° phase difference for each direction and three different directions are image-processed to make one resolution enhancement by increasing the optical transfer function in three directions. In this paper, we present a novel cross structured-illumination confocal microscope (CSICM) that takes the advantage of the technology of both SIM and the confocal microscope. The CSICM uses only two directions with three phase difference images, for a total of six images. By reducing the number of images that must be obtained, the total image acquisition time and image reconstruction time in obtaining the final output images can be decreased, and the confocal microscope provides axial information of the sample automatically. We demonstrate our method of cross illumination and evaluate the performance of the CSICM and compare it to the conventional SIM and the confocal microscope.
- URI
- http://iopscience.iop.org/article/10.1088/0957-0233/23/10/105403/metahttp://hdl.handle.net/20.500.11754/67318
- ISSN
- 0957-0233
- DOI
- 10.1088/0957-0233/23/10/105403
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > ELECTRICAL AND BIOMEDICAL ENGINEERING(전기·생체공학부) > Articles
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML