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dc.contributor.author이규용-
dc.date.accessioned2018-04-15T22:35:48Z-
dc.date.available2018-04-15T22:35:48Z-
dc.date.issued2011-01-
dc.identifier.citationBrain research, Vol.1370 No.- [2011], 53-63en_US
dc.identifier.issn0006-8993-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0006899310025345?via%3Dihub-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/67098-
dc.description.abstractNeurotoxic effects have been suggested for L-3,4-dihydroxyphenylalanine (L-DOPA), while neuroprotective effects have been proposed for statins. We investigated whether certain statins (simvastatin or pitavastatin) could inhibit L-DOPA neurotoxicity. Neuronally-differentiated PC12 (nPC12) cells were treated with L-DOPA and/or statins for 24 h, and their viabilities were measured using a cell counting kit, trypan blue staining, and 4',6-diamidino-2-phenylindole (DAPI) staining. Free radical and specific intracellular signaling protein levels were measured with 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) and Western blotting, respectively. High concentrations of L-DOPA reduced nPC12 cell viability, but combined treatment with statins restored viability. Treatment with 200 mu M L-DOPA increased free radical and hydroxyl radical levels, but combined treatment with 5 mu M statins decreased these levels. Survival-related signaling proteins were decreased in nPC12 cells treated with 200 mu M L-DOPA, but combined treatment with 5 mu M statins significantly increased the levels of these proteins. Treatment with 200 mu M L-DOPA significantly increased death-related signaling proteins, while combined treatment with 5 mu M statins reduced the levels of these proteins. Pretreatment with LY294002, a phosphatidylinositol 3 kinase (PI3K) inhibitor, before combined treatment with statins and L-DOPA almost completely blocked the protective effects of statins. These results indicate that statins reduce L-DOPA neurotoxicity by lowering oxidative stress and by enhancing survival signals and inhibiting death signals via activation of the PI3K pathway. (C) 2010 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipThis work was supported by a grant from the Research Fund ofHanyang University (HY-2010-MC).en_US
dc.language.isoenen_US
dc.publisherELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDSen_US
dc.subjectL-DOPAen_US
dc.subjectStatinen_US
dc.subjectPhosphatidylinositol 3-kinaseen_US
dc.subjectNeurotoxicityen_US
dc.titleProtective effects of statins on L-DOPA neurotoxicity due to the activation of phosphatidylinositol 3-kinase and free radical scavenging in PC12 cell cultureen_US
dc.typeArticleen_US
dc.relation.volume1370-
dc.identifier.doi10.1016/j.brainres.2010.11.021-
dc.relation.page53-63-
dc.relation.journalBRAIN RESEARCH-
dc.contributor.googleauthorKoh, Seong-Ho-
dc.contributor.googleauthorPark, Hyun-Hee-
dc.contributor.googleauthorChoi, Na-Young-
dc.contributor.googleauthorLee, Kyu-Yong-
dc.contributor.googleauthorKim, Sangjae-
dc.contributor.googleauthorLee, Young Joo-
dc.contributor.googleauthorKim, Hee-Tae-
dc.relation.code2011201421-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF MEDICINE[S]-
dc.sector.departmentDEPARTMENT OF MEDICINE-
dc.identifier.pidkylee-
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COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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