Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 주재범 | - |
dc.date.accessioned | 2018-04-14T09:32:20Z | - |
dc.date.available | 2018-04-14T09:32:20Z | - |
dc.date.issued | 2011-01 | - |
dc.identifier.citation | In Biosensors and Bioelectronics 2011 26(5):2135-2141 | en_US |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0956566310006342?via%3Dihub | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/66116 | - |
dc.description.abstract | This paper reports a highly reproducible immunoassay of cancer markers using surface-enhanced Raman scattering (SERS) imaging. SERS is a highly sensitive detection method but it is limited in its ability to achieve reproducible signal enhancement because of the difficulty with precisely controlling the uniform distribution of hot junctions. Consequently, inconsistent enhancement prevents the wide exploitation of SERS detection as a bio-detection tool for quantitative analysis. To resolve this problem, we explored the use of a SERS imaging-based immunoassay. For this purpose, Raman reporter-labeled hollow gold nanospheres (HGNs), were manufactured and antibodies were immobilized onto their surfaces for targeting specific antigens. After the formation of sandwich immunocomplexes using these functional HGNs on the surfaces of gold patterned wells, the SERS mapping images were measured. For target protein markers, 12 x 9 pixels were imaged using a Raman mapping technique in the 0-10(-4) g/mL concentration range, and the SERS signals for 66 pixels were averaged. Here, the SERS imaging-based assay shows much better correlations between concentration and intensity than does the conventional point-based assay. The limits of detection were determined to be 0.1 pg/mL and 1.0 pg/mL for angiogenin (ANG) and alpha-fetoprotein (AFP), respectively. This detection sensitivity is increased by three or four orders of magnitude over that of conventional ELISA method. The detectable dynamic range for SERS imaging (10(-4)-10(-12) g/mL) is also much wider than that for ELISA (10(-6)-10(-9) g/mL). (C) 2010 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by the National Research Foundationof Korea (Grant Numbers R11-2009-044-1002-0 andK20904000004-09A0500-00410), the National Cancer Center ofKorea (Grant 0620400-1) and the Seoul Research and BusinessDevelopment Program (Grant No. 10574). This work was also partiallysupported by the Ministry of Knowledge Economy (MKE)and Korea Industrial Technology Foundation (KOTEF) through theHuman Resource Training Project for Strategic Technology. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY, OXFORD FULFILLMENT CENTRE THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND | en_US |
dc.subject | Hollow gold nanosphere | en_US |
dc.subject | Surface-enhanced Raman scattering imaging | en_US |
dc.subject | Biomarker | en_US |
dc.subject | Immunoassay | en_US |
dc.subject | Microarray chip | en_US |
dc.subject | NANOPARTICLE PROBES | en_US |
dc.subject | PROTEIN | en_US |
dc.subject | NANOSPHERES | en_US |
dc.subject | CELLS | en_US |
dc.subject | NANOSTRUCTURES | en_US |
dc.subject | ANGIOGENIN | en_US |
dc.subject | SUBSTRATE | en_US |
dc.subject | ANTIGEN | en_US |
dc.subject | ASSAY | en_US |
dc.title | Highly reproducible immunoassay of cancer markers on a gold-patterned microarray chip using surface-enhanced Raman scattering imaging | en_US |
dc.type | Article | en_US |
dc.relation.volume | 26 | - |
dc.identifier.doi | 10.1016/j.bios.2010.09.021 | - |
dc.relation.page | 2135-2141 | - |
dc.relation.journal | BIOSENSORS & BIOELECTRONICS | - |
dc.contributor.googleauthor | Lee, Moonkwon | - |
dc.contributor.googleauthor | Lee, Sangyeop | - |
dc.contributor.googleauthor | Lee, Jung-hwan | - |
dc.contributor.googleauthor | Lim, Hyun-woo | - |
dc.contributor.googleauthor | Seong, Gi Hun | - |
dc.contributor.googleauthor | Lee, Eun Kyu | - |
dc.contributor.googleauthor | Chang, Soo-Ik | - |
dc.contributor.googleauthor | Oh, Chil Hwan | - |
dc.contributor.googleauthor | Choo, Jaebum | - |
dc.relation.code | 2011201344 | - |
dc.sector.campus | S | - |
dc.sector.daehak | GRADUATE SCHOOL[S] | - |
dc.sector.department | DEPARTMENT OF BIONANOTECHNOLOGY | - |
dc.identifier.pid | jbchoo | - |
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