146 0

Full metadata record

DC FieldValueLanguage
dc.contributor.author최호순-
dc.date.accessioned2018-04-11T14:47:22Z-
dc.date.available2018-04-11T14:47:22Z-
dc.date.issued2011-12-
dc.identifier.citationLiver International, 2011, 31(9), P.1315-1324en_US
dc.identifier.issn1478-3223-
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/abs/10.1111/j.1478-3231.2011.02602.x-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/65597-
dc.description.abstractBACKGROUND:Mitochondria are the main sites for fatty acid oxidation and play a central role in lipotoxicity and nonalcoholic steatohepatitis.AIMS:We investigated whether carnitine prevents free fatty acid (FFA)-induced lipotoxicity in vitro and in vivo.METHODS:HepG2 cells were incubated with FFA, along with carnitine and carnitine complexes. Mitochondrial β-oxidation, transmembrane potential, intracellular ATP levels and changes in mitochondrial copy number and morphology were analysed. Otsuka Long-Evans Tokushima Fatty and Long-Evans Tokushima Otsuka rats were segregated into three experimental groups and fed for 8 weeks with (i) normal chow, (ii) a methionine choline-deficient (MCD) diet or (iii) an L-carnitine-supplemented MCD diet.RESULTS:Carnitine prevented FFA-induced apoptosis (16% vs. 3%, P < 0.05). FFA treatment resulted in swollen mitochondria with increased inner matrix density and loss of cristae. However, mitochondria co-treated with carnitine had normal ultrastructure. The mitochondrial DNA copy number was higher in the carnitine treatment group than in the palmitic acid treatment group (375 vs. 221 copies, P < 0.05). The carnitine group showed higher mitochondrial β-oxidation than did the control and palmitic acid treatment groups (597 vs. 432 and 395 ccpm, P < 0.05). Carnitine treatment increased the mRNA expression of carnitine palmitoyltransferase 1A and peroxisome proliferator-activated receptor-γ, and carnitine-lipoic acid further augmented the mRNA expression. In the in vivo model, carnitine-treated rats showed lower alanine transaminase levels and lesser lobular inflammation than did the MCD-treated rats.CONCLUSIONS:Carnitine and carnitine-lipoic acid prevent lipotoxicity by increasing mitochondrial β-oxidation and reducing intracellular oxidative stress.en_US
dc.language.isoenen_US
dc.publisherBlackwell Publishing Ltden_US
dc.subjectcarnitineen_US
dc.subjectlipotoxicityen_US
dc.subjectmitochondriaen_US
dc.titlePrevention of free fatty acid-induced hepatic lipotoxicity by carnitine via reversal of mitochondrial dysfunctionen_US
dc.typeArticleen_US
dc.relation.no9-
dc.relation.volume31-
dc.identifier.doi10.1111/j.1478-3231.2011.02602.x-
dc.relation.page1315-1324-
dc.relation.journalLIVER INTERNATIONAL-
dc.contributor.googleauthorJunJun, D. W.Cho, W. K.Jun, J. H.Kwon, H. J.Jang, K. S.Kim, H. J.Jeon, H. J.Lee, K. N.Lee, H. L.Lee, O. Y. D. W.-
dc.contributor.googleauthorCho, W. K.-
dc.contributor.googleauthorJun, J. H.-
dc.contributor.googleauthorKwon, H. J.-
dc.contributor.googleauthorJang, K. S.-
dc.contributor.googleauthorKim, H. J.-
dc.contributor.googleauthorJeon, H. J.-
dc.contributor.googleauthorLee, K. N.-
dc.contributor.googleauthorLee, H. L.-
dc.contributor.googleauthorLee, O. Y.-
dc.relation.code2011211589-
dc.sector.campusS-
dc.sector.daehakCOLLEGE OF MEDICINE[S]-
dc.sector.departmentDEPARTMENT OF MEDICINE-
dc.identifier.pidhschoi96-
Appears in Collections:
COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE