Generation of multipotent induced neural crest by direct reprogramming of human postnatal fibroblasts with a single transcription factor
- Title
- Generation of multipotent induced neural crest by direct reprogramming of human postnatal fibroblasts with a single transcription factor
- Author
- 오요한
- Keywords
- 키워드없음
- Issue Date
- 2014-10
- Publisher
- Elsevier
- Citation
- CELL STEM CELL, 15권, 4호, pp.497-506
- Abstract
- Neural crest (NC) generates diverse lineages including peripheral neurons, glia, melanocytes, and mesenchymal derivatives. Isolating multipotent human NC has proven challenging, limiting our ability to understand NC development and model NC-associated disorders. Here, we report direct reprogramming of human fibroblasts into induced neural crest (iNC) cells by overexpression of a single transcription factor, SOX10, in combination with environmental cues including WNT activation. iNC cells possess extensive capacity for migration in vivo, and single iNC clones can differentiate into the fourmain NC lineages. We further identified a cell surface marker for prospective isolation of iNCs, which was used to generate and purify iNCs from familial dysautonomia (FD) patient fibroblasts. FD-iNC cells displayed defects in cellular migration and alternative mRNA splicing, providing insights into FD pathogenesis. Thus, this study provides an accessible platform for studying NC biology and disease through rapid and efficient reprogramming of human postnatal fibroblasts.
- URI
- http://www.cell.com/cell-stem-cell/fulltext/S1934-5909(14)00309-9http://hdl.handle.net/20.500.11754/58306
- ISSN
- 1934-5909; 1875-9777
- DOI
- 10.1016/j.stem.2014.07.013
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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