SERS-based immunoassay of tumor marker VEGF using DNA aptamers and silica-encapsulated hollow gold nanospheres
- Title
- SERS-based immunoassay of tumor marker VEGF using DNA aptamers and silica-encapsulated hollow gold nanospheres
- Author
- 주재범
- Keywords
- ENHANCED RAMAN-SCATTERING; PATTERNED MICROARRAY CHIP; CANCER MARKERS; NANOPROBES; SENSORS; CELLS; LUNG; BIOSENSORS; SYSTEM; GROWTH
- Issue Date
- 2013-02
- Publisher
- Royal Society of Chemistry
- Citation
- Physical chemistry chemical physics, Feb 2013, 15(15), P.5379-5385
- Abstract
- A novel SERS-based sandwich immunoassay using DNA aptamers, silica-encapsulated hollow gold nanospheres (SEHGNs) and a gold-patterned microarray was developed for sensitive detection of VEGF (vascular endothelial growth factor) angiogenesis protein markers. Here, a DNA aptamer conjugated to SEHGN was used as a highly reproducible SERS-encoding nanoprobe, and a hybrid microarray including hydrophilic gold wells and other hydrophobic areas was used as a SERS substrate. Target specific DNA aptamers that fold into a G-quadruplex structure were used as a target recognition unit instead of VEGF antibodies. The detection sensitivity was increased by 2 or 3 orders of magnitude over the conventional ELISA method. In particular, the dynamic concentration range was 3 or 4 orders of magnitude greater than that of conventional ELISA. The results demonstrate that this sensing strategy using DNA aptamers is a powerful platform for the design of novel immune-sensors with high performance. In particular, SERS-based detection using SEHGNs provides great promise for highly sensitive biomarker sensing with unprecedented advantages.
- URI
- http://pubs.rsc.org/en/Content/ArticleLanding/2013/CP/C2CP43155F#!divAbstracthttp://hdl.handle.net/20.500.11754/57589
- ISSN
- 1463-9076
- DOI
- 10.1039/c2cp43155f
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > ETC
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