Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 김정목 | - |
dc.date.accessioned | 2018-04-03T06:52:47Z | - |
dc.date.available | 2018-04-03T06:52:47Z | - |
dc.date.issued | 2013-06 | - |
dc.identifier.citation | Life Sciences, 2013, 92(22), P.1064-1071 | en_US |
dc.identifier.issn | 0024-3205 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S002432051300218X?via%3Dihub | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/56920 | - |
dc.description.abstract | Aims: We previously demonstrated that the novel guggulsterone derivative guggulsterone-52 (GG-52) inhibited the activation of nuclear factor (NF)-kappa B signaling in intestinal epithelial cells and had preventive and therapeutic effects on dextran sulfate sodium-induced acute colitis. This study investigates the anti-inflammatory effects of GG-52 on bone marrow-derived dendritic cells (BMDCs) and chronic colitis in IL-10(-/-) mice. Main methods: BMDCs were generated from the femurs of C57BL/6 wild-type and IL-10(-/-) mice. BMDCs were stimulated with lipopolysaccharide (LPS) in the presence or absence of GG-52. The effect of GG-52 on NF-kappa B signaling in BMDCs was examined by real-time RT-PCR for IL-12p40 and TNF-alpha gene expression, western blotting for I kappa B alpha degradation, and electrophoretic mobility shift assay. For in vivo studies, wildtype or IL-10(-/-) mice were treated with or without GG-52. Colitis was quantified by the evaluation of histopathological findings. Double immunofluorescence staining for CD11c and phosphorylated I kappa B kinase (IKK)-alpha was performed to detect IKK activation in DCs in colonic tissue. Key findings: GG-52 significantly inhibited LPS-induced IL-12p40 and TNF-alpha gene expression, I kappa B alpha degradation, and NF-kappa B DNA binding activity in BMDCs. In the IL-10(-/-) mouse model chronic colitis, administration of GG-52 significantly reduced the severity of colitis as assessed by histopathology, and suppressed IKK activation in DCs in colonic tissue. Significance: These results indicate that the novel guggulsterone derivative GG-52 blocks NF-kappa B activation in BMDCs and ameliorates chronic colitis in IL-10(-/-) mice, which suggest that GG-52 is a potential therapeutic agent for inflammatory bowel diseases. (C) 2013 Elsevier Inc. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by a grant of the Korea Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea (Program No. A110485). | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier Science LTD | en_US |
dc.subject | Guggulsterone | en_US |
dc.subject | NF-κB | en_US |
dc.subject | Dendritic cells, | en_US |
dc.subject | Inflammatory bowel diseases | en_US |
dc.title | The guggulsterone derivative GG-52 inhibits NF-kappa B signaling in bone marrow-derived dendritic cells and attenuates colitis in IL-10 knockout mice | en_US |
dc.type | Article | en_US |
dc.relation.no | 22 | - |
dc.relation.volume | 92 | - |
dc.identifier.doi | 10.1016/j.lfs.2013.04.003 | - |
dc.relation.page | 1064-1071 | - |
dc.relation.journal | LIFE SCIENCES | - |
dc.contributor.googleauthor | Kang, Seung Joo | - |
dc.contributor.googleauthor | Kim, Jung Mogg | - |
dc.contributor.googleauthor | Koh, Seong-Joon | - |
dc.contributor.googleauthor | Kim, Su Hyun | - |
dc.contributor.googleauthor | Im, Jong Pil | - |
dc.contributor.googleauthor | Jung, Hyun Chae | - |
dc.contributor.googleauthor | Kim, Joo Sung | - |
dc.relation.code | 2013011158 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jungmogg | - |
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