Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 황정욱 | - |
dc.date.accessioned | 2018-04-03T04:02:53Z | - |
dc.date.available | 2018-04-03T04:02:53Z | - |
dc.date.issued | 2014-06 | - |
dc.identifier.citation | NUCLEIC ACIDS RESEARCH; 2014, 42 11, p6999-p7011, 13p. | en_US |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | https://academic.oup.com/nar/article/42/11/6999/1452617 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/56016 | - |
dc.description.abstract | Requiem (REQ/DPF2) was originally identified as an apoptosis-inducing protein in mouse myeloid cells and belongs to the novel Kruppel-type zinc finger d4-protein family of proteins, which includes neuro-d4 (DPF1) and cer-d4 (DPF3). Interestingly, when a portion of the REQ messenger ribonucleic acid (mRNA) 3' untranslated region (3'UTR), referred to as G8, was overexpressed in K562 cells, beta-globin expression was induced, suggesting that the 3'UTR of REQ mRNA plays a physiological role. Here, we present evidence that the REQ mRNA 3'UTR, along with its trans-acting factor, Staufen1 (STAU1), is able to reduce the level of REQ mRNA via STAU1-mediated mRNA decay (SMD). By screening a complementary deoxyribonucleic acid (cDNA) expression library with an RNA-ligand binding assay, we identified STAU1 as an interactor of the REQ mRNA 3'UTR. Specifically, we provide evidence that STAU1 binds to putative 30-nucleotide stem-loop-structured RNA sequences within the G8 region, which we term the protein binding site core; this binding triggers the degradation of REQ mRNA and thus regulates translation. Furthermore, we demonstrate that siRNA-mediated silencing of either STAU1 or UPF1 increases the abundance of cellular REQ mRNA and, consequently, the REQ protein, indicating that REQ mRNA is a target of SMD. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Oxford University Press | en_US |
dc.subject | complementary DNA | en_US |
dc.subject | messenger RNA | en_US |
dc.subject | nuclear protein | en_US |
dc.subject | requiem | en_US |
dc.subject | RNA | en_US |
dc.subject | small interfering RNA | en_US |
dc.subject | unclassified drug | en_US |
dc.title | Staufen1-mediated mRNA decay induces Requiem mRNA decay through binding of Staufen1 to the Requiem 3'UTR | en_US |
dc.type | Article | en_US |
dc.relation.no | 11 | - |
dc.relation.volume | 42 | - |
dc.identifier.doi | 10.1093/nar/gku388 | - |
dc.relation.page | 6999-7011 | - |
dc.relation.journal | NUCLEIC ACIDS RESEARCH | - |
dc.contributor.googleauthor | Hwang, Jung-wook | - |
dc.contributor.googleauthor | Kim, Min-Young | - |
dc.contributor.googleauthor | Lee, Jong-Joo | - |
dc.contributor.googleauthor | Ha, Dae-Hyun | - |
dc.contributor.googleauthor | Kim, Jong-hwan | - |
dc.contributor.googleauthor | Kim, Chul-Geun | - |
dc.contributor.googleauthor | Park, Jun-gyun | - |
dc.contributor.googleauthor | Hwang, Jung-wook | - |
dc.contributor.googleauthor | Kim, Chan-Gil | - |
dc.relation.code | 2014036861 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jwhwang | - |
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