DOPAMINE NEURONS; IN-VITRO; MICROGLIAL ACTIVATION; NEURAL PRECURSORS; HUMAN BLASTOCYSTS; RAT MODEL; DIFFERENTIATION; MINOCYCLINE; DERIVATION; ISCHEMIA
Stem cell reports, 2014, 3(2), P.353-364
We here report that doxycycline, an antibacterial agent, exerts dramatic effects on human embryonic stem and induced pluripotent stem cells (hESC/iPSCs) survival and self-renewal. The survival-promoting effect was also manifest in cultures of neural stem cells (NSCs) derived from hESC/iPSCs. These doxycycline effects are not associated with its antibacterial action, but mediated by direct activation of a PI3K-AKT intracellular signal. These findings indicate doxycycline as a useful supplement for stem cell cultures, facilitating their growth and maintenance.