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dc.contributor.author김상성-
dc.date.accessioned2018-03-30T00:49:49Z-
dc.date.available2018-03-30T00:49:49Z-
dc.date.issued2016-05-
dc.identifier.citationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v. 475, No. 1, Page. 44-50en_US
dc.identifier.issn0006-291X-
dc.identifier.issn1090-2104-
dc.identifier.urihttps://www.sciencedirect.com/science/article/pii/S0006291X16307124-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/54062-
dc.description.abstractBackground: Rosmarinic acid (RA) is a polyphenolic ester of caffeic acid and is commonly found in the Nepetoideae subfamily of flowering mint plants. Because RA has previously exhibited antioxidant, neuroprotective, and antidepressant-like effects, we evaluated its influences on cellular functions in neuronal cultures. Objective: To elucidate possible mechanisms of RA, we investigated the influences of acute RA administration on long-term potentiation (LTP), plasticity-related protein expression, and scopolamine-induced cell death in organotypic hippocampal slice cultures. Methods: LTP analysis in organotypic hippocampal slice cultures (OHSCs) was carried out with various ion channel blockers, such as AP5 (10 mu M), CNQX (10 mu M), niflumic acid (100 mu M), and scopolamine (300 mu M) in response to RA (1, 10 or 100 mu g/mL) treatment. Protein expression and cell death assays in the presence of scopolamine were examined to observe the effects of RA. For LTP analysis, baseline field excitatory postsynaptic potentials (fEPSPs) were recorded in CA1 by a 60-channel multielectrode array (MEA) every min for 40 min before 15 min of high-frequency stimulation (HFS) to the Schaffer collaterals and commissural pathways, followed by a successive 50 min of recording. For protein expression measurements, anti-BDNF and anti-GluR2 antibodies were used for Western blotting assays in whole-hippocampal tissue homogenate. Finally, for cell death assays, OHSCs were exposed to a culture medium containing propidium iodide (PI) for 24 or 48 h, followed by the assessment of cell death by fluorescent image analysis of PI uptake. Results: and discussion: Our results indicate that RA treatment enhances fEPSPs following HFS in CA1 synapses at 1 and 10 mu g/ml RA, an effect that was inhibited by CNQX and NFA but not by AP5. RA treatment also increases the expression of BDNF and GluR-2 proteins and prevents cell death of scopolamine-exposed OHSCs. Our results suggest the possibility that rosmarinic acid can enhance neural plasticity by modulating glutamatergic signaling pathways, as well as providing neuroprotection with reduced cholinergic activity. (C) 2016 Published by Elsevier Inc.en_US
dc.description.sponsorshipThis work was supported by the National Research Foundation of Korea (NRF) of Korea (2014R1A2A1A09006320 and 2015R1D1A1A02062097) and Hanyang University (201500000000138).en_US
dc.language.isoen_USen_US
dc.publisherACADEMIC PRESS INC ELSEVIER SCIENCEen_US
dc.subjectRosmarinic acid (RA)en_US
dc.subjectLong-term potentiation (LTP)en_US
dc.subjectMultielectrode array (MEA)en_US
dc.subjectNeuronal plasticityen_US
dc.subjectNeuroprotectionen_US
dc.subjectHippocampusen_US
dc.titleAcute rosmarinic acid treatment enhances long-term potentiation, BDNF and GluR-2 protein expression, and cell survival rate against scopolamine challenge in rat organotypic hippocampal slice culturesen_US
dc.typeArticleen_US
dc.relation.no1-
dc.relation.volume475-
dc.identifier.doi10.1016/j.bbrc.2016.04.153-
dc.relation.page44-50-
dc.relation.journalBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS-
dc.contributor.googleauthorHwang, Eun-Sang-
dc.contributor.googleauthorKim, Hyun-Bum-
dc.contributor.googleauthorChoi, Ga-Young-
dc.contributor.googleauthorLee, Seok-
dc.contributor.googleauthorLee, Sung-Ok-
dc.contributor.googleauthorKim, SangSeong-
dc.contributor.googleauthorPark, Ji-Ho-
dc.relation.code2016001086-
dc.sector.campusE-
dc.sector.daehakCOLLEGE OF PHARMACY[E]-
dc.sector.departmentDEPARTMENT OF PHARMACY-
dc.identifier.pidtalpiot-
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COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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