Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김정목 | - |
dc.date.accessioned | 2018-03-29T01:25:09Z | - |
dc.date.available | 2018-03-29T01:25:09Z | - |
dc.date.issued | 2014-12 | - |
dc.identifier.citation | WORLD JOURNAL OF GASTROENTEROLOGY, 20(45), p.17084-17091 | en_US |
dc.identifier.issn | 1007-9327 | - |
dc.identifier.issn | 2219-2840 | - |
dc.identifier.uri | https://www.wjgnet.com/1007-9327/full/v20/i45/17084.htm | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/53537 | - |
dc.description.abstract | AIM: To investigate the effect of Clostridium difficile (C. difficile) infection in an interleukin 10-deficient (IL-10(-/-)) mouse model of inflammatory bowel disease.METHODS: Bone marrow-derived dendritic cells isolated from wild type (WT) and IL-10(-/-) mice were stimulated for 4 h with C. difficile toxin A (200 mu g/mL), and gene expression of interferon (IFN)-gamma, IL-12 and IL-23 was determined by real-time reverse transcription polymerase chain reaction. WT and IL-10(-/-) mice (n = 20 each) were exposed to an antibiotic cocktail for three days and then were injected with clindamycin (i.p.). Mice (n = 10 WT, 10 IL-10(-/-)) were then challenged with oral administration of C. difficile (1 x 10(5) colony forming units of strain VPI 10463). Animals were monitored daily for 7 d for signs of colitis. Colonic tissue samples were evaluated for cytokine gene expression and histopathologic analysis.RESULTS: C. difficile toxin A treatment induced IFN-gamma gene expression to a level that was significantly higher in BDMCs from IL-10(-/-) compared to those from WT mice (P < 0.05). However, expression of IL-12 and IL-23 was not different among the groups. Following C. difficile administration, mice developed diarrhea and lost weight within 2-3 d. Weight loss was significantly greater in IL-10(-/-) compared to WT mice (P < 0.05). C. difficile infection induced histopathologic features typical of colitis in both IL-10(-/-) and WT mice. The histopathologic severity score was significantly higher in the IL-10(-/)- than in WT mice (mean +/- standard error; 5.50 +/- 0.53 vs 2.44 +/- 0.46; P < 0.05). This was accompanied by a significantly greater increase in IFN-gamma gene expression in colonic tissues from IL-10(-/-) than from WT mice challenged with C. difficile (P < 0.05).CONCLUSION: These results indicate that colitis is more severe after C. difficile infection in IL-10(-/-) mice, and that IFN-gamma expression is involved in this process. (C) 2014 Baishideng Publishing Group Inc. All rights reserved. | en_US |
dc.language.iso | en | en_US |
dc.publisher | BAISHIDENG PUBLISHING GROUP INC | en_US |
dc.subject | Clostridium difficile | en_US |
dc.subject | Inflammatory bowel disease | en_US |
dc.subject | Interleukin 10-deficient mice | en_US |
dc.subject | Interferon-gamma | en_US |
dc.subject | Colitis | en_US |
dc.title | Clostridium difficile infection aggravates colitis in interleukin 10-deficient mice | en_US |
dc.type | Article | en_US |
dc.relation.volume | 20 | - |
dc.identifier.doi | 10.3748/wjg.v20.i45.17084 | - |
dc.relation.page | 17084-17091 | - |
dc.relation.journal | WORLD JOURNAL OF GASTROENTEROLOGY | - |
dc.contributor.googleauthor | Kim, Mi Na | - |
dc.contributor.googleauthor | Koh, Seong-Joon | - |
dc.contributor.googleauthor | Kim, Jung Mogg | - |
dc.contributor.googleauthor | Im, Jong Pil | - |
dc.contributor.googleauthor | Jung, Hyun Chae | - |
dc.contributor.googleauthor | Kim, Joo Sung | - |
dc.relation.code | 2014040901 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | jungmogg | - |
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