Identification of a novel cell-penetrating peptide from human phosphatidate phosphatase LPIN3
- Title
- Identification of a novel cell-penetrating peptide from human phosphatidate phosphatase LPIN3
- Author
- 최제민
- Keywords
- CPP; drug delivery system; LPIN; PTD; ALLERGIC AIRWAY INFLAMMATION; PROTEIN TRANSDUCTION; GENE DELIVERY; IN-VIVO; CYTOPLASMIC DOMAIN; THERAPEUTICS; ACTIVATION; MECHANISM
- Issue Date
- 2012-12
- Publisher
- SPRINGER
- Citation
- Molecules and cells, 2012, 34(6), 577p~582p
- Abstract
- Biomolecules such as proteins, DNA, and RNA are macromolecules and can not cross the cell membrane. However, cell-penetrating peptide (CPP) has been shown to deliver therapeutic biomolecules successfully into cells. The various and widely used CPPs including TAT, VP22, and Antp are mostly non-human originated CPPs, and are limited by their potential toxicity and immunogenicity. We report here on a newly identified novel cell-penetrating sequence (LPIN; RRKRRRRRK) from the nuclear localization sequence (NLS) of human nuclear phosphatase, LPIN3. LPIN-EGFP recombinant protein was concentration- and time-dependently delivered into cells and localized to the nucleus as well as the cytoplasm. It penetrated the cell membrane by lipid raft-mediated endocytosis by binding to heparan sulfate proteoglycan. LPIN-EGFP was successfully delivered into primary mouse splenocytes in vitro and it could be delivered into various tissues including liver, kidney, and intestine in mice after intra-peritoneal injection. This research suggests that LPIN-CPP could be used in a drug delivery system to deliver therapeutic biomolecules including peptides, proteins, DNA, and RNA and without the limitations of non-human originated CPPs such as TAT-CPP.
- URI
- https://link.springer.com/article/10.1007%2Fs10059-012-0284-yhttp://hdl.handle.net/20.500.11754/53069
- ISSN
- 1016-8478
- DOI
- 10.1007/s10059-012-0284-y
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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