Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최동호 | - |
dc.date.accessioned | 2018-03-27T01:59:25Z | - |
dc.date.available | 2018-03-27T01:59:25Z | - |
dc.date.issued | 2013-04 | - |
dc.identifier.citation | Journal of the Korean Surgical Society, 2013, 84(4), P.202-208 | en_US |
dc.identifier.issn | 2233-7903 | - |
dc.identifier.uri | https://synapse.koreamed.org/DOIx.php?id=10.4174/jkss.2013.84.4.202 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/52886 | - |
dc.description.abstract | Purpose: Primary mammalian hepatocytes largely retain their liver-specific functions when they are freshly derived from donors. However, long-term cultures of functional hepatocytes are difficult to establish. To increase the longevity and maintain the differentiated functions of hepatocytes in primary culture, cells can be cultured in a sandwich configuration of collagen. In such a configuration, hepatocytes can be cultured for longer periods compared with cultures on single layers of collagen. However, research regarding mouse hepatocytes in sandwich culture is lacking. Methods: Primary mouse hepatocytes were sandwiched between two layers of collagen to maintain the stability of their liver-specific functions. After gelation, 2 mL of hepatocyte culture medium was applied. Results: After 24 hours, 5, 10 days of culture, the collagen gel sandwich maintained the cellular border and numbers of bile canaliculi more efficiently than a single collagen coating in both high and low density culture dishes. Reverse transcription-polymerase chain reaction analysis of alpha-l-antitrypsin (AAT), hepatocyte nuclear factor 4 alpha (HNF4A), alphafetoprotein, albumin, tryptophan oxygenase (TO), the tyrosine aminotransferase gene, glucose-6-phosphatase, glyceraldehyde-3- phosphate dehydrogenase for mouse primary hepatocytes cultured on collagen coated dishes and collagen gels showed superior hepatocyte-related gene expression in cells grown using the collagen gel sandwich culture system. AAT, HNF4A, albumin, TO were found to be expressed in mouse hepatocytes cultured on collagen gels for 5 and 10 days. In contrast, mouse hepatocytes grown on collagen-coated dishes did not express these genes after 5 and 10 days of culture. Conclusion: The collagen gel sandwich method is suitable for primary culture system of adult mouse hepatocytes. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Korean Surgical Society | en_US |
dc.subject | Collagen | en_US |
dc.subject | Culture | en_US |
dc.subject | Hepatocyte | en_US |
dc.title | Successful mouse hepatocyte culture with sandwich collagen gel formation | en_US |
dc.type | Article | en_US |
dc.relation.volume | 84 | - |
dc.identifier.doi | 10.4174/jkss.2013.84.4.202 | - |
dc.relation.page | 202-208 | - |
dc.relation.journal | JOURNAL OF THE KOREAN SURGICAL SOCIETY | - |
dc.contributor.googleauthor | Choi, Hyun Jung | - |
dc.contributor.googleauthor | Choi, Dongho | - |
dc.relation.code | 2013005612 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | crane87 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.