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dc.contributor.author김연제-
dc.date.accessioned2018-03-26T08:09:00Z-
dc.date.available2018-03-26T08:09:00Z-
dc.date.issued2013-04-
dc.identifier.citationApplied Microbiology and Biotechnology, Jun 2013, 97(12), P.5627-5634en_US
dc.identifier.issn0175-7598-
dc.identifier.urihttp://link.springer.com/article/10.1007%2Fs00253-013-4879-9-
dc.identifier.urihttp://hdl.handle.net/20.500.11754/52619-
dc.description.abstractIn this study, a novel system for synthesis of 2-butanone from levulinic acid (gamma-keto-acid) via an enzymatic reaction was developed. Acetoacetate decarboxylase (AADC; E.C. 4.1.1.4) from Clostridium acetobutylicum was selected as a biocatalyst for decarboxylation of levulinic acid. The purified recombinant AADC from Escherichia coli successfully converted levulinic acid to 2-butanone with a conversion yield of 8.4-90.3 % depending on the amount of AADC under optimum conditions (30 A degrees C and pH 5.0) despite that acetoacetate, a beta-keto-acid, is a natural substrate of AADC. In order to improve the catalytic efficiency, an AADC-mediator system was tested using methyl viologen, methylene blue, azure B, zinc ion, and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as mediators. Among them, methyl viologen showed the best performance, increasing the conversion yield up to 6.7-fold in comparison to that without methyl viologen. The results in this study are significant in the development of a renewable method for the synthesis of 2-butanone from biomass-derived chemical, levulinic acid, through enzymatic decarboxylation.en_US
dc.description.sponsorshipThis research was supported by the Korean Ministry of Knowledge and Economy (2009301009001B) and the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (NRF-2009-C1AAA001-0093286). We thank Prof. Young Je Yoo and Mr. Hoe Suk Lee at Seoul National University for their help in molecular docking simulation.en_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.subjectEnzymatic decarboxylationen_US
dc.subjectAcetoacetate decarboxylaseen_US
dc.subjectLevulinic aciden_US
dc.subject2-Butanoneen_US
dc.titleConversion of levulinic acid to 2-butanone by acetoacetate decarboxylase from Clostridium acetobutylicumen_US
dc.typeArticleen_US
dc.relation.volume97-
dc.relation.page5627-5634-
dc.relation.journalAPPLIED MICROBIOLOGY AND BIOTECHNOLOGY-
dc.contributor.googleauthorMin, Kyoungseon-
dc.contributor.googleauthorKim, Seil-
dc.contributor.googleauthorYum, Taewoo-
dc.contributor.googleauthorKim, Yunje-
dc.contributor.googleauthorSang, Byoung-In-
dc.contributor.googleauthorUm, Youngsoon-
dc.contributor.googleauthor김경선-
dc.contributor.googleauthor김세일-
dc.contributor.googleauthor염태우-
dc.contributor.googleauthor김윤제-
dc.contributor.googleauthor상봉인-
dc.contributor.googleauthor엄영순-
dc.relation.code2013008970-
dc.sector.campusS-
dc.sector.daehakRESEARCH INSTITUTE[S]-
dc.sector.departmentRESEARCH INSTITUTE OF INDUSTRIAL SCIENCE-
dc.identifier.pidyjkim4070-
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