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Effect of Resistance Training on Skeletal Muscle Gene Expression in Rats: a Beadarray Analysis

Title
Effect of Resistance Training on Skeletal Muscle Gene Expression in Rats: a Beadarray Analysis
Author
오상덕
Keywords
Resistance training; beadarray; gene expression
Issue Date
2013-02
Publisher
한국생명과학회
Citation
생명과학회지 Journal of Life Science, Feb 2013, 23(1), P.116-124
Abstract
The aim was to examine resistance exercise-related genes after 8 weeks of resistance training. Thirty-two male Sprague-Dawley rats were divided into four groups: 4 weeks sedentary (4 wks CON, n=8), 8 weeks sedentary (8 wks CON, n=8), 4 weeks exercise training (4 wks REG, n=8), and 8 weeks exercise training (8 wks REG, n=8). The rats were trained to climb a 1-m vertical incline (85-degree), with weights secured to their tails. They climbed 10 times, 3 days per week, for 8 consecutive weeks. Skeletal muscle was taken from the flexor halucis longus after the exercise training. After separating the total RNA, large-scale gene expression was investigated by beadarray (Illumina RatRef-12 Expression BeadChip) analysis, and qPCR was used to inspect the beadarray data and to analyze the RNA quantitatively. The detection p-value for the genes was p log2 (condition)- log2 (reference)} was >1.0, and the DiffScore was >20. In total, the expression of 30 genes significantly increased 4 weeks after the exercise training, and the expression of six genes decreased. At 8 weeks, the expression of five genes significantly increased and that of 12 decreased. Several genes are potentially involved in resistance exercise and muscle hypertrophy, including 1) regulation of cell growth (IGFBP1, PLA2G2A, OKL38); 2) myogenesis (CSRP3); 3) tissue regeneration and muscle development (MUSTN1, MYBPH); 4) hypertrophy (CYR61, ATF3, NR4A3); and 5) glucose metabolism (G6PC, PCK1). These results may help to explain previously reported physiological changes of the skeletal muscle and suggest new avenues for further investigation.본 연구의 목적은 저항성 운동 후 골격근에서 저항성 관련 유전자를 규명하는 것이다. 연구 목적을 달성하기 위하여 총 32두의 Sprague-Dawley계 수컷 흰쥐를 분양 받은 후 4주차 통제군(4 wks CON, n=8), 8주차 통제군(8 wks CON, n=8), 4주차 운동군(4 wks REG, n=8), 8주차 운동군(8 wks REG, n=8)으로 집단을 분류하였다. 저항성 운동군은 꼬리에 무게를 달고 동물용 사다리(1-m vertical, 85 degree incline)를 오르는 저항성 사다리 운동을 1회 10번, 주당 3일, 4주와 8주간 점증적으로 실시하였으며, 골격근 조직은 저항성 운동 후 장무지굴근(flexor hallucis longus; FHL)을 적출하여 분석에 이용하였다. 적출한 골격근에서 total RNA를 분류한 후, 대규모 유전자 발현분석을 위하여 Illumina RatRef-12 Expression BeadChip을 이용한 Beadarray를 시행하였으며, Beadarray 결과를 확인하기 위해 qPCR (real-time quantitative PCR)를 실시하였다. 유의성 검증은 Beadstudio software를 이용하여 실시하였으며, Beadarray 데이터 중 Detection p-value to log2 (condition)- log2 (reference)} to >1.0, DiffScore to >20인 유전자만을 통계적으로 의미 있는 유전자로 선택하였다. 4주차 저항성 운동 후 통제집단에 비해 2배 이상 유의하게 발현이 증가한 유전자는 30개였으며, 6개의 유전자가 감소하였다. 8주차 저항성 운동 후에는 5개의 유전자가 발현이 증가하였으며, 12개의 유전자가 유의하게 감소하였다. 연구결과 다음의 유전자를 포함한 저항성 운동과 근비대와 관련 후보 유전자를 도출하였다; 1) 세포 성장 조절(IGFBP1, PLA2G2A, OKL38); 2) 근육발생(CSRP3); 3) 조직 재생과 근육 발달(MUSTN1, MYBPH); and 4) 비대 모델(CYR61, ATF3, NR4A3); and 5) 당대사(G6PC, PCK1). 이러한 연구결과는 차후 저항성 운동과 관련된 다양한 생리학적 변인을 연구하는데 있어서 기초 자료를 제공할 것으로 생각된다.
URI
http://www.koreascience.or.kr/article/ArticleFullRecord.jsp?cn=SMGHBM_2013_v23n1_116http://hdl.handle.net/20.500.11754/51816
ISSN
1225-9918; 2287-3406
DOI
10.5352/JLS.2013.23.1.116
Appears in Collections:
COLLEGE OF ART AND PHYSICAL EDUCATION[S](예술·체육대학) > ETC
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