Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이민형 | - |
dc.date.accessioned | 2018-03-24T01:28:19Z | - |
dc.date.available | 2018-03-24T01:28:19Z | - |
dc.date.issued | 2013-10 | - |
dc.identifier.citation | JOURNAL OF CONTROLLED RELEASE, 2013, 171(1), P.1-10 | en_US |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.uri | https://ac.els-cdn.com/S0168365913003805/1-s2.0-S0168365913003805-main.pdf?_tid=6db6873e-d722-4ea9-b129-5d55cdb67485&acdnat=1521115046_eb25d7ebdffd0b6ccef91a5ca78f35ca | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/51704 | - |
dc.description.abstract | Gene therapy is suggested as a promising alternative strategy of hepatocellular carcinoma (HCC, also called hepatoma) therapy. To achieve a successful and safe gene therapy, tight regulation of gene expression is required to minimize side-effects in normal tissues. In this study, we developed a novel hypoxia and hepatoma dual specific gene expression vector. The constructed vectors were transfected into various cell lines using bio-reducible polymer, PAM-ABP. First, pAFPS-Luc or pAFPL-Luc vector was constructed with the alpha-fectoprotein (AFP) promoter and enhancer for hepatoma tissue specific gene expression. Then, pEpo-AFPL-Luc was constructed by insertion of the erythropoietin (Epo) enhancer for hypoxic cancer specific gene expression. In vitro transfection assay showed that pEpo-AFPL-Luc transfected hepatoma cell increased gene expression under hypoxic condition. To confirm the therapeutic effect of dual specific vector, herpes simplex virus thymidine kinase (HSV-TK) gene was introduced for cancer cell killing. The pEpo-AFPL-TK was transfected into hepatoma cell lines in the presence of ganciclovir (GCV) pro-drug. Caspase-3/7, MTT and TUNEL assays elucidated that pEpo-AFPL-TK transfected cells showed significant increasing of death rate in hypoxic hepatoma cells compared to controls. Therefore, the hypoxia/hepatoma dual specific gene expression vector with the Epo enhancer and AFP promoter may be useful for hepatoma specific gene therapy. (C) 2013 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by the NIH Grant CA107070 (S.W. Kim) and grants from the National Research Foundation in Korea funded by the Ministry of Education, Science and Technology 2012K001394 (M. Lee) and Basic Science Research Program 2012R1A6A3A03040715 (H.A. Kim). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS | en_US |
dc.subject | Hepatoma | en_US |
dc.subject | Gene regulation | en_US |
dc.subject | Suicide gene therapy | en_US |
dc.subject | Bio-reducible polymer | en_US |
dc.subject | Cancer hypoxia | en_US |
dc.subject | ALPHA-FETOPROTEIN PROMOTER | en_US |
dc.subject | SELECTIVE CANCER-THERAPY | en_US |
dc.subject | VIRUS THYMIDINE KINASE | en_US |
dc.subject | RECURRENT GLIOBLASTOMA | en_US |
dc.subject | PHASE-I | en_US |
dc.subject | HYPOXIA | en_US |
dc.subject | VECTOR | en_US |
dc.subject | MYOCARDIUM | en_US |
dc.subject | HEPATOMA | en_US |
dc.subject | TARGET | en_US |
dc.title | Hypoxia/hepatoma dual specific suicide gene expression plasmid delivery using bio-reducible polymer for hepatocellular carcinoma therapy | en_US |
dc.title.alternative | hepatoma dual specific suicide gene expression plasmid delivery using bio-reducible polymer for hepatocellular carcinoma therapy | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 171 | - |
dc.identifier.doi | 10.1016/j.jconrel.2013.06.033 | - |
dc.relation.page | 1-10 | - |
dc.relation.journal | JOURNAL OF CONTROLLED RELEASE | - |
dc.contributor.googleauthor | Kim, Hyun Ah | - |
dc.contributor.googleauthor | Nam, Kihoon | - |
dc.contributor.googleauthor | Lee, Minhyung | - |
dc.contributor.googleauthor | Kim, Sung Wan | - |
dc.relation.code | 2013010611 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | minhyung | - |
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